Laboratory of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang Street, Shenyang 110142, People's Republic of China.
Eur J Med Chem. 2011 Jan;46(1):160-7. doi: 10.1016/j.ejmech.2010.10.031. Epub 2010 Nov 3.
Two novel dinuclear complexes Co(2)(L)(2)(phen)(2)·2H(2)O (1), Co(2)(L)(2)(bipy)(2) (2) (where phen=1,10-phenanthroline, bipy=2,2'-bipyridine, and L=biphenyl-2,4'-dicarboxylic acid) have been synthesized and characterized using IR, (1)H NMR, element analysis and single-crystal X-ray diffractometry. The binding of the complexes with HC-DNA (HeLa cells DNA, which was extracted by ourselves) was investigated by fluorescence spectrum. The experimental results show that the two complexes have the ability to bind with extracted HC-DNA. Gel electrophoresis assay demonstrates the ability of the complexes to cleave the extracted HC-DNA. The complexes exhibit a higher cytotoxicity against tumor cells as against normal cells in vitro. Further more, the apoptotic tests indicate that the complexes have an apoptotic effect on HeLa cells.
合成并表征了两种新型双核配合物Co(2)(L)(2)(phen)(2)·2H(2)O (1)和Co(2)(L)(2)(bipy)(2) (2)（其中 phen=1,10-菲咯啉，bipy=2,2'-联吡啶，L=联苯-2,4'-二羧酸），使用红外光谱、（1）H NMR、元素分析和单晶 X 射线衍射法进行了表征。通过荧光光谱研究了配合物与 HC-DNA（我们自己提取的 HeLa 细胞 DNA）的结合情况。实验结果表明，两种配合物均具有与提取的 HC-DNA 结合的能力。凝胶电泳实验证明了配合物具有切割提取的 HC-DNA 的能力。体外实验表明，与正常细胞相比，这些配合物对肿瘤细胞具有更高的细胞毒性。此外，凋亡实验表明这些配合物对 HeLa 细胞具有凋亡作用。
