Laboratory of Coordination Chemistry, International Key Laboratory of Shenyang Inorganic Molecule-Based Chemicals, Shenyang University of Chemical Technology, Shenyang, P R China.
Dalton Trans. 2012 Aug 28;41(36):11187-94. doi: 10.1039/c2dt30990d.
Two complexes Pd(L(1))(en)·4H(2)O (1), [Pd(L(2))(en)]·2H(2)O (2), (L(1) = pyridine-2,3-dicarboxylic acid, L(2) = pyrazine-2,3-dicarboxylic acid, and en = ethylenediamine) have been synthesized and characterized using IR, (1)H NMR and element analysis. X-ray analyses of complexes 1 and 2 revealed that 1 belongs to the triclinic system with space group P1(2), and 2 belongs to the monoclinic system with space group P2(1)/n. The binding of the complexes with HC-DNA (HeLa cells DNA, which was extracted by ourselves) were measured by fluorescence spectrum and viscometry. Gel electrophoresis assay demonstrates the ability of the complexes to cleave the extracted HC-DNA. The complexes exhibit a higher cytotoxicity against tumor cells as against normal cells in vitro. Further more, apoptotic tests indicate that the complexes have an apoptotic effect on HeLa cells.
两个配合物Pd(L(1))(en)·4H(2)O(1)，[Pd(L(2))(en)]·2H(2)O(2)（L(1)=吡啶-2,3-二羧酸，L(2)=吡嗪-2,3-二羧酸，en=乙二胺）被合成并通过红外光谱（IR）、（1）H NMR 和元素分析进行了表征。配合物 1 和 2 的 X 射线分析表明，1 属于三斜晶系，空间群为 P1(2)，2 属于单斜晶系，空间群为 P2(1)/n。配合物与 HC-DNA（HeLa 细胞 DNA，由我们自己提取）的结合通过荧光光谱和粘度计进行了测量。凝胶电泳试验表明，该配合物能够切割提取的 HC-DNA。该配合物在体外对肿瘤细胞的细胞毒性高于对正常细胞的细胞毒性。此外，凋亡试验表明该配合物对 HeLa 细胞具有凋亡作用。
