Food Technology Research Center, Hiroshima Prefectural Technology Research Institute, Hijiyama-Honmachi 12-70, Minami-ku, Hiroshima 732-0816, Japan.
J Biosci Bioeng. 2011 Mar;111(3):312-9. doi: 10.1016/j.jbiosc.2010.10.011. Epub 2010 Nov 19.
To avoid the inhibition of methane production by ammonia that occurs during the degradation of garbage, anaerobic digestion with prior ammonia production and subsequent stripping was investigated. In the ammonia production phase, the maximum ammonia concentration was approximately 2800 mg N/kg of total wet sludge in the range of 4 days of sludge retention time, indicating that only 43% of total nitrogen in the model garbage was converted to ammonia. The model garbage from which ammonia was produced and stripped was subjected to semi-continuous thermophilic dry anaerobic digestion over 180 days. The gas yield was in the range of 0.68 to 0.75 Nm(3)/kg volatile solid, and it decreased with the decrease of the sludge retention time. The ammonia-nitrogen concentration in the sludge was kept below 3000 mg N/kg total wet sludge. Microbial community structure analysis revealed that the phylum Firmicutes dominated in the ammonia production, but the community structure changed at different sludge retention times. In dry anaerobic digestion, the dominant bacteria shifted from the phylum Thermotogae to Firmicutes. The dominant archaeon was the genus Methanothermobacter, but the ratio of Methanosarcina increased during the process of dry anaerobic digestion.
为避免在垃圾降解过程中氨对甲烷生成的抑制作用，研究了先进行氨生产，然后进行汽提的厌氧消化。在氨生产阶段，污泥停留时间为 4 天时，氨的最大浓度约为 2800mgN/kg 总湿污泥，表明模型垃圾中只有 43%的总氮转化为氨。从已生产和汽提氨的模型垃圾中，进行了 180 天的半连续嗜热干厌氧消化。产气量在 0.68 到 0.75Nm(3)/kg 挥发性固体之间，随着污泥停留时间的减少而减少。污泥中的氨氮浓度保持在 3000mgN/kg 总湿污泥以下。微生物群落结构分析表明，在氨生产过程中厚壁菌门占优势，但在不同的污泥停留时间下，群落结构发生了变化。在干厌氧消化中，从Thermotogae 门到Firmicutes 门的优势细菌发生了转变。优势古菌是 Methanothermobacter 属，但在干厌氧消化过程中，Methanosarcina 的比例增加。
