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鸡粪干式厌氧氨-甲烷生产

Dry anaerobic ammonia-methane production from chicken manure.

作者信息

Abouelenien Fatma, Kitamura Yoshiaki, Nishio Naomichi, Nakashimada Yutaka

机构信息

Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Kagamiyama 1-3-1, Higashi-Hiroshima 739-8530, Japan.

出版信息

Appl Microbiol Biotechnol. 2009 Mar;82(4):757-64. doi: 10.1007/s00253-009-1881-3. Epub 2009 Jan 31.

Abstract

The effect of temperature on production of ammonia during dry anaerobic fermentation of chicken manure (CM), inoculated with thermophilic methanogenic sludge, was investigated in a batch condition for 8 days. Incubation temperature did not have a significant effect on the production of ammonia. Almost complete inhibition of production of methane occurred at 55 and 65 degrees C while quite low yields of 8.45 and 6.34 ml g(-1) VS (volatile solids) were observed at 35 and 45 degrees C due to a higher accumulation of ammonia. In order to improve the production of methane during dry anaerobic digestion of CM, stripping of ammonia was performed firstly on the CM previously fermented at 65 degrees C for 8 days: the stripping for 1 day at 85 degrees C and pH 10 removed 85.5% of ammonia. The first-batch fermentation of methane for 75 days was conducted next, using the ammonia-stripped CM inoculated with methanogenic sludge at different ratios, (CM: thermophilic sludge) of 1:2, 1:1, and 2:1 on volume per volume basis at both 35 and 55 degrees C. Production of methane improved and was higher than that of the control (without stripping of ammonia) but the yield of 20.4 ml g(-1) VS was still low, so second stripping of ammonia was conducted, which resulted in 74.7% removal of ammonia. A great improvement in the production of methane of 103.5 ml g(-1) VS was achieved during the second batch for 55 days.

摘要

在间歇条件下研究了温度对接种嗜热产甲烷污泥的鸡粪(CM)干式厌氧发酵过程中氨产生的影响,为期8天。培养温度对氨的产生没有显著影响。在55℃和65℃时,甲烷产生几乎完全受到抑制,而在35℃和45℃时,由于氨的积累较高,观察到相当低的产率,分别为8.45和6.34 ml g(-1)VS(挥发性固体)。为了提高CM干式厌氧消化过程中的甲烷产量,首先对预先在65℃发酵8天的CM进行氨汽提:在85℃和pH 10下汽提1天可去除85.5%的氨。接下来进行第一批甲烷发酵75天,使用以不同比例(CM:嗜热污泥)1:2、1:1和2:1(体积比)接种产甲烷污泥的脱氨CM,在35℃和55℃下进行。甲烷产量有所提高且高于对照(未进行氨汽提),但20.4 ml g(-1)VS的产率仍然较低,因此进行了第二次氨汽提,氨去除率达74.7%。在第二批55天的发酵过程中,甲烷产量大幅提高,达到103.5 ml g(-1)VS。

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