Department of Biology, Bates College, Lewiston, ME 04240 USA.
Respir Physiol Neurobiol. 2011 Feb 15;175(2):220-7. doi: 10.1016/j.resp.2010.11.007. Epub 2010 Nov 19.
Chronic exposure to hyperoxia alters the postnatal development and innervation of the rat carotid body. We hypothesized that this plasticity is related to changes in the expression of neurotrophic factors or related proteins. Rats were reared in 60% O(2) from 24 to 36h prior to birth until studied at 3d of age (P3). Protein levels for brain-derived neurotrophic factor (BDNF) were significantly reduced (-70%) in the P3 carotid body, while protein levels for its receptor, tyrosine kinase B, and for glial cell line-derived neurotrophic factor (GDNF) were unchanged. Transcript levels in the carotid body were downregulated for the GDNF receptor Ret (-34%) and the neuropeptide Vgf (-67%), upregulated for Cbln1 (+205%), and unchanged for Fgf2; protein levels were not quantified for these genes. Immunohistochemical analysis revealed that Vgf and Cbln1 proteins are expressed within the carotid body glomus cells. These data suggest that BDNF, and perhaps other neurotrophic factors, contribute to abnormal carotid body function following perinatal hyperoxia.
慢性高氧暴露会改变大鼠颈动脉体的出生后发育和神经支配。我们假设这种可塑性与神经营养因子或相关蛋白表达的变化有关。在出生前的 24 至 36 小时内，将大鼠饲养在 60%的氧气中，直到出生后 3 天（P3）进行研究。P3 颈动脉体中的脑源性神经营养因子（BDNF）蛋白水平显著降低（-70%），而其受体酪氨酸激酶 B 和胶质细胞系衍生的神经营养因子（GDNF）的蛋白水平不变。颈动脉体中的转录水平下调了 GDNF 受体 Ret（-34%）和神经肽 Vgf（-67%），上调了 Cbln1（+205%），而 Fgf2 不变；这些基因的蛋白水平没有被量化。免疫组织化学分析显示，Vgf 和 Cbln1 蛋白在颈动脉体的小球细胞内表达。这些数据表明，BDNF 以及其他神经营养因子可能与围产期高氧后颈动脉体功能异常有关。
