Department of Orthopaedics, University of Duisburg-Essen, and Department of Pathology, University Hospital of Essen, Pattbergstrasse 1-3, 45239 Essen, Germany.
Acta Biomater. 2011 Mar;7(3):1364-8. doi: 10.1016/j.actbio.2010.11.016. Epub 2010 Nov 19.
Particle-induced osteolysis is a major cause of aseptic loosening after total joint replacement. The purpose of the current study was to evaluate cellular senescence of macrophages and giant cells in patients with aseptic hip loosening by determination of SA-β-Gal (SA-β-galactosidase), a reliable and frequently used indicator of cellular senescence. The level of senescence in capsule and interface membranes was significantly higher in patients with aseptic loosening in comparison to specimens from patients without aseptic loosening. Using Spearman's rank correlation, we found that the expression of SA-β-Gal in giant cells (p=0.002) and macrophages (p=0.050) in the interface membranes correlates significantly with the degree of polyethylene debris. We speculate that the induction of DNA damage by wear particles is responsible for premature senescence. Consequently, we conclude that the form of senescence observed in this study is a "stress-induced senescence".
颗粒诱导性骨溶解是全关节置换术后无菌性松动的主要原因。本研究旨在通过检测 SA-β-Gal（β-半乳糖苷酶）来评估无菌性髋关节松动患者的巨噬细胞和巨细胞的细胞衰老情况，SA-β-Gal 是细胞衰老的可靠且常用的指标。与无无菌性松动患者的标本相比，无菌性松动患者的囊和界面膜中的衰老水平明显更高。通过 Spearman 等级相关分析，我们发现界面膜中巨细胞（p=0.002）和巨噬细胞（p=0.050）中 SA-β-Gal 的表达与聚乙烯碎屑的程度显著相关。我们推测磨损颗粒诱导的 DNA 损伤是导致过早衰老的原因。因此，我们得出结论，本研究中观察到的衰老形式是“应激诱导性衰老”。
