17β-雌二醇核壳型分子印迹聚合物在硅胶纳米粒子表面的研究。
The study of core-shell molecularly imprinted polymers of 17β-estradiol on the surface of silica nanoparticles.
机构信息
School of Pharmacy, Nanjing Medical University, Hanzhong Road, Nanjing 210029, China.
出版信息
Biosens Bioelectron. 2011 Jan 15;26(5):2791-5. doi: 10.1016/j.bios.2010.10.045. Epub 2010 Oct 31.
In this paper, highly selective core-shell molecularly imprinted polymers of 17β-estradiol on the surface of silica nanoparticles (SiO(2)@E2-MIPs) were prepared. The SiO(2)@E2-MIPs were characterized by Fourier transform infrared spectrometer (FT-IR), transmission electron microscope (TEM), dynamic adsorption and static adsorption tests. The sorption capacity of the SiO(2)@E2-MIPs were nearly 5 times that of the non-imprinted polymers (NIPs), and it only took 25 min to achieve the sorption equilibrium. It indicated that the SiO(2)@E2-MIPs exhibited a high selectivity, large adsorption capacity and fast kinetics. When the SiO(2)@E2-MIPs were used as dispersive solid-phase extraction (dSPE) absorbents to selectively enrich and determine estrogens in duck feed, the average recoveries of E2 and estriol (E3) were higher than 96.74% and 72.07%, respectively, and the relative standard deviations (RSD) of E2 and E3 were less than 1.61% and 3.28%, respectively. The study provides an effective method for the separation and enrichment of estrogens in the complex matrix samples by the SiO(2)@E2-MIPs.
本文制备了在硅胶纳米粒子（SiO2@E2-MIPs）表面的高选择性的 17β-雌二醇的核壳型分子印迹聚合物。通过傅里叶变换红外光谱（FT-IR）、透射电子显微镜（TEM）、动态吸附和静态吸附实验对 SiO2@E2-MIPs 进行了表征。SiO2@E2-MIPs 的吸附容量接近非印迹聚合物（NIPs）的 5 倍，仅需 25 min 即可达到吸附平衡。这表明 SiO2@E2-MIPs 表现出高选择性、大吸附容量和快速动力学。当 SiO2@E2-MIPs 用作分散固相萃取（dSPE）吸附剂，以选择性地富集和测定鸭饲料中的雌激素时，E2 和雌三醇（E3）的平均回收率均高于 96.74%和 72.07%，E2 和 E3 的相对标准偏差（RSD）均小于 1.61%和 3.28%。该研究为通过 SiO2@E2-MIPs 从复杂基质样品中分离和富集雌激素提供了一种有效的方法。
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