School of Pharmacy, Nanjing Medical University, Hanzhong Road 140, Nanjing 210029, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Sep 1;879(25):2595-600. doi: 10.1016/j.jchromb.2011.07.017. Epub 2011 Jul 20.
This paper reports a surface molecular self-assembly strategy for molecular imprinting on magnetic nanoparticles for selective separation and detection of estrogens in feeds. First, γ-methacryloxypropyltrimethoxysilane (MEMO) was successfully assembled at the surface magnetic nanoparticles through simple free radical polymerization, and subsequently, the copolymerization was further assembled between methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA) in the presence of templates 17β-estradiol (E2). The synthesized magnetic molecularly imprinted polymers for E2 (E2-MMIPs) showed quick separation, large adsorption capacity, high selectivity and fast binding kinetics for E2. Meanwhile, a dispersive solid-phase extraction (DSPE) based on E2-MMIPs has been established for efficient separation and fast enrichment of estrogens from the feeds. The assay exhibited a linear range of 0.1-4 μM for E2 and estriol (E3) with the correlation coefficient above 0.9996 and 0.9994, respectively. Recoveries of E2 from three kinds of feeds spiked at different concentration levels ranged from 92.7% to 97.0% with RSD<4.7%, and recoveries of E3 ranged from 71.9% to 83.1% with RSD<4.9%, respectively. The method is simple and sensitive, and can be used as an alternative tool to effectively separate and enrich the trace of estrogens in agricultural products by HPLC-UV.
本文报道了一种表面分子自组装策略，用于在磁性纳米粒子上进行分子印迹，以选择性分离和检测饲料中的雌激素。首先，通过简单的自由基聚合，成功地将γ-甲基丙烯酰氧基丙基三甲氧基硅烷（MEMO）组装在磁性纳米粒子表面，随后在模板 17β-雌二醇（E2）的存在下，将甲基丙烯酸（MAA）和乙二醇二甲基丙烯酸酯（EGDMA）进一步共聚组装。合成的用于 E2 的磁性分子印迹聚合物（E2-MMIPs）对 E2 具有快速分离、大吸附容量、高选择性和快速结合动力学。同时，建立了基于 E2-MMIPs 的分散固相萃取（DSPE）方法，用于从饲料中有效分离和快速富集雌激素。该测定法对 E2 和雌三醇（E3）的线性范围分别为 0.1-4 μM，相关系数均大于 0.9996 和 0.9994。从三种不同浓度水平添加的饲料中提取 E2 的回收率在 92.7%至 97.0%之间，RSD<4.7%，E3 的回收率在 71.9%至 83.1%之间，RSD<4.9%。该方法简单灵敏，可用于高效分离和富集农产品中痕量雌激素的替代工具，通过 HPLC-UV 进行。
