Cellular Autoimmunity Unit, Department of Clinical Sciences in Malmö, Lund University, Malmö University Hospital, Malmö, Sweden.
Biochem Biophys Res Commun. 2011 Jan 7;404(1):16-22. doi: 10.1016/j.bbrc.2010.11.024. Epub 2010 Nov 19.
Many microRNAs (miRNAs) are known to be cell-type specific and are implicated in development of diseases. We investigated the global expression pattern of miRNAs in human pancreatic islets compared to liver and skeletal muscle, using bead-based technology and quantitative RT-PCR. In addition to the known islet-specific miR-375, we also found enrichment of miR-127-3p, miR-184, miR-195 and miR-493∗ in the pancreatic islets. The expression of miR-375, miR-127-3p, miR-184 and the liver-enriched miR-122 is positively correlated to insulin biosynthesis, while the expression of miR-127-3p and miR-184 is negatively correlated to glucose-stimulated insulin secretion (GSIS). These correlations were absent in islets of glucose intolerant donors (HbA1c ≥ 6.1). We suggest that the presence of an islet-specific miRNA network, which consists of at least miR-375, miR-127-3p and miR-184, potentially involved in insulin secretion. Our results provide new insight into miRNA-mediated regulation of insulin secretion in healthy and glucose intolerant subjects.
许多 microRNAs（miRNAs）被认为是细胞类型特异性的，并与疾病的发生有关。我们使用基于珠粒的技术和定量 RT-PCR 研究了与人肝和骨骼肌相比，miRNAs 在人胰岛中的全局表达模式。除了已知的胰岛特异性 miR-375 外，我们还发现 miR-127-3p、miR-184、miR-195 和 miR-493∗在胰岛中富集。miR-375、miR-127-3p、miR-184 和富含肝的 miR-122 的表达与胰岛素生物合成呈正相关，而 miR-127-3p 和 miR-184 的表达与葡萄糖刺激的胰岛素分泌（GSIS）呈负相关。在葡萄糖不耐受供体（HbA1c≥6.1）的胰岛中不存在这些相关性。我们认为，至少由 miR-375、miR-127-3p 和 miR-184 组成的胰岛特异性 miRNA 网络可能参与胰岛素分泌。我们的结果为 miRNA 介导的健康和葡萄糖不耐受个体胰岛素分泌的调节提供了新的见解。
