Infectious Diseases Program, Lovelace Respiratory Research Institute, Albuquerque, NM 87108, USA.
FEBS Lett. 2011 Jan 3;585(1):128-32. doi: 10.1016/j.febslet.2010.11.023. Epub 2010 Nov 19.
Acetylated microtubules (AcMTs), a post-translationally modified form of microtubules, promote polarized protein transport. Here we report that influenza A virus (IAV) induces the acetylation of microtubules in epithelial cells. By employing specific inhibitors and siRNA we demonstrate Rho GTPase-mediated downregulation of tubulin deacetylase activity in IAV-infected cells, resulting in increased tubulin acetylation. Further, we demonstrate that depolymerization/deacetylation or enhanced acetylation of microtubules decreased or increased, respectively, the release of virions from infected cells. IAV assembly requires the polarized delivery of viral components to apical plasma membrane. Our findings suggest the potential involvement of AcMTs in polarized trafficking of IAV components.
乙酰化微管（AcMTs）是微管的一种翻译后修饰形式，可促进蛋白的极化运输。本文报道甲型流感病毒（IAV）可诱导上皮细胞中微管的乙酰化。通过使用特异性抑制剂和 siRNA，我们证明了 Rho GTPase 介导的 IAV 感染细胞中微管去乙酰化酶活性下调，导致微管乙酰化增加。此外，我们还证明微管的解聚/去乙酰化或乙酰化增强分别降低或增加了感染细胞中病毒粒子的释放。IAV 的组装需要病毒成分的极化递送到顶质膜。我们的研究结果表明，AcMTs 可能参与了 IAV 成分的极化运输。
