Jichen Q V, Chen G, Jiang G, Ding J, Gao W, Chen C, Shen Z
Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.
Transplant Proc. 2010 Nov;42(9):3759-62. doi: 10.1016/j.transproceed.2010.06.040.
To validate the use of pig-to-monkey transplantation as an experimental animal model for study of the induction of immune suppression of cardiac xenografts via intrathymic inoculation with xenogeneic spleen cells and whole-body γ-irradiation.
Donors (Meishan pigs) and recipients (Rhesus monkeys) were randomized into 3 groups. Group 2 was the control group; group 2 received whole-body irradiation, and group 3 received whole-body irradiation and intrathymic inoculation. In each group, survival time of heterotopic heart xenografts in monkeys were observed. A mixed lymphocyte reaction assay with (3)H thymidine was used to examine changes in lymphocyte function in all groups on the day of transplantation. In each group, recipient serum was harvested for analysis of IL-2 and IL-10 concentrations.
Mean (SD) survival time of donor hearts in group 3 was significantly longer (91.1 [22.8] hours) than in group 1 (36.6 [5.8] hours) (P < .01). The results of mixed lymphocyte reaction assay demonstrated a significant reduction in the stimulation index in group 3 compared with groups 1 and 2 (P < .01) when recipient splenocytes responded to the donor stimulator. The IL-2 concentration in recipients in all groups was much higher during rejection of xenografts than before transplantation (P < .01).
These results suggest that pretreatment with intrathymic inoculation or whole-body irradiation induces T-cell immunosuppression. The IL-2 concentration is closely correlated with xenograft rejection.
验证猪到猴的移植作为一种实验动物模型，用于研究通过胸腺内接种异种脾细胞和全身γ射线照射诱导心脏异种移植物免疫抑制的情况。
将供体（梅山猪）和受体（恒河猴）随机分为3组。第2组为对照组；第2组接受全身照射，第3组接受全身照射和胸腺内接种。观察每组中猴体内异位心脏异种移植物的存活时间。在移植当天，采用含3H胸腺嘧啶核苷的混合淋巴细胞反应试验检测所有组淋巴细胞功能的变化。每组采集受体血清分析白细胞介素-2（IL-2）和白细胞介素-10（IL-10）浓度。
第3组供体心脏的平均（标准差）存活时间（91.1 [22.8]小时）明显长于第1组（36.6 [5.8]小时）（P <.01）。混合淋巴细胞反应试验结果表明，当受体脾细胞对供体刺激细胞作出反应时，第3组的刺激指数与第1组和第2组相比显著降低（P <.01）。在异种移植物排斥期间，所有组受体的IL-2浓度均比移植前高得多（P <.01）。
这些结果表明，胸腺内接种或全身照射预处理可诱导T细胞免疫抑制。IL-2浓度与异种移植物排斥密切相关。
