Laboratory of Microbiology and Immunology, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.
Virus Res. 2011 Jan;155(1):343-8. doi: 10.1016/j.virusres.2010.11.005. Epub 2010 Nov 21.
Bovine leukemia virus (BLV) infection of cattle has been increasing yearly in Japan although several European countries have successfully eradicated the infection. In the present study, phylogenetic analysis on the env gene obtained from 64 tumor samples found in different regions in Japan was carried out in order to define the genetic background of BLV strains prevailing in the country. Most of the Japanese isolates were found to reside in the consensus cluster or genotype 1 of BLV strains (Rodriguez et al., 2009). Out of them, 21 isolates and 10 isolates exhibited the identical sequences, respectively. Only one isolate was classified into the different genotype related to the US isolates. Analysis on the deduced amino acids of gp51 demonstrated the sequence diversity in the neutralizing domain. These data may indicate that two major populations of BLV prevailed throughout Japan, whereas antigenic variants also exist. It was further proved that multiple invasion of the genetically different BLV strains have occurred in Japan.
牛白血病病毒（BLV）感染在日本逐年增加，尽管一些欧洲国家已经成功根除了这种感染。在本研究中，对从日本不同地区的 64 个肿瘤样本中获得的 env 基因进行了系统进化分析，以确定在该国流行的 BLV 株的遗传背景。大多数日本分离株被发现存在于 BLV 株的共识簇或基因型 1 中（Rodriguez 等人，2009 年）。其中，21 个分离株和 10 个分离株分别具有相同的序列。只有一个分离株被归类为与美国分离株相关的不同基因型。对 gp51 的推导氨基酸分析表明，在中和域存在序列多样性。这些数据可能表明，两种主要的 BLV 种群在日本流行，而抗原变异体也存在。进一步证明，遗传上不同的 BLV 株在日本已经多次入侵。
