Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan.
Vet Microbiol. 2010 Dec 15;146(3-4):366-70. doi: 10.1016/j.vetmic.2010.05.026. Epub 2010 May 27.
Chlamydophila felis is a causative agent of acute and chronic conjunctivitis and pneumonia in cats. Cats can be vaccinated with killed or attenuated C. felis. However, current serodiagnostics cannot distinguish these cats from naturally infected cats. This causes difficulty of early diagnosis and seroepidemiological survey for C. felis. We previously reported that C. felis CF0218 can be used as a C. felis-infection-specific diagnostic antigen in experimentally infected and/or vaccinated cats. In this study, we evaluated an enzyme-linked immunosorbent assay using recombinant CF0218 as antigen (CF0218-ELISA) to detect anti-C. felis antibody in 714 sera of domestic cats whose histories of vaccination against C. felis are known. The 44 vaccinated cats were 93% negative using CF0218-ELISA; half of these scored positive by immunofluorescence assay (IFA) using C. felis-infected cells as antigen. The 670 non-vaccinated cats had CF0218-ELISA positivity rates that were statistically in agreement with IFA (18% vs. 21%). These results show that CF0218, which was identified as a C. felis-infection-specific antigen, is a useful serodiagnostic antigen to distinguish naturally C. felis-infected cats from vaccinated and non-infected cats.
猫衣原体是引起猫急性和慢性结膜炎和肺炎的病原体。猫可以用灭活或减毒的猫衣原体进行疫苗接种。然而，目前的血清诊断方法无法区分这些猫与自然感染的猫。这给猫衣原体的早期诊断和血清流行病学调查带来了困难。我们之前曾报道过，猫衣原体 CF0218 可用作实验感染和/或接种猫的衣原体感染特异性诊断抗原。在这项研究中，我们评估了使用重组 CF0218 作为抗原的酶联免疫吸附试验（CF0218-ELISA），以检测 714 份已知有猫衣原体疫苗接种史的家猫血清中的抗衣原体抗体。44 只接种疫苗的猫使用 CF0218-ELISA 的阴性率为 93%；其中一半通过使用感染猫衣原体的细胞作为抗原的免疫荧光测定（IFA）呈阳性。670 只未接种疫苗的猫的 CF0218-ELISA 阳性率与 IFA 具有统计学一致性（18%比 21%）。这些结果表明，CF0218 作为一种衣原体感染特异性抗原，是一种有用的血清学诊断抗原，可区分自然感染的猫与接种疫苗和未感染的猫。
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