基于重组酶介导的扩增检测方法检测.
A Recombinase-Aided Amplification Assay for the Detection of .
机构信息
Shanghai Animal Disease Prevention and Control Center, Shanghai, China.
School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an, China.
出版信息
Pol J Microbiol. 2023 Sep 20;72(3):339-343. doi: 10.33073/pjm-2023-029. eCollection 2023 Sep 1.
is an important zoonotic agent for humans and various animals. A recombinase-aided amplification (RAA) assay was developed for detecting . RAA can be performed in a closed tube at 39°C within 30 min. The detection limit was 10.6 copies of the plasmid DNA per reaction. No positive signals for other pathogens were detected. The coincidence rate of RAA and conventional PCR was 95.24% (20/21) and 100% (96/96) for positive and negative samples, respectively. The established RAA assay is a simple, rapid, highly sensitive, and specific method for detecting .
是一种重要的人畜共患病病原体。本研究建立了一种用于检测 的重组酶辅助扩增（RAA）检测方法。RAA 可以在 39°C 下在封闭管中 30 分钟内完成。检测限为每个反应 10.6 拷贝 的质粒 DNA。未检测到其他病原体的阳性信号。RAA 与常规 PCR 的符合率分别为阳性和阴性样本的 95.24%（20/21）和 100%（96/96）。该研究建立的 RAA 检测方法是一种简单、快速、高度敏感和特异的检测 方法。
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