Smith R, Kapatsa J C, Rosenbaum B A, Adams L G
Department of Veterinary Pathology, College of Veterinary Medicine, Texas A&M University, College Station 77843-4463.
Am J Vet Res. 1990 Apr;51(4):512-7.
Bovine T-cell lines reactive with Brucella abortus were established by repeated stimulation with B abortus and mitomycin C-treated autologous antigen-presenting cells. Representative results were obtained, using 33 cell lines from 14 cows. Cultures responded to the virulent laboratory strain 2308, the vaccine strain 19, and the rough mutant strain RB51 in thymidine-incorporation assays. The cells in these cultures required antigen-presenting cells for their response to B abortus. Autologous antigen-presenting cells were optimal for most lines tested, although some T-cell lines could respond to B abortus in the presence of some, but not all, allogeneic antigen-presenting cells. The cell lines expressed cell surface markers characteristics of activated bovine T cels. Of the cell lines tested for expression of cluster-determinant (CD) 4 and CD8 cell surface antigens, no cells in any cultures expressed the bovine CD8 equivalent, but all cultures included CD4+ cells in variable amounts. Some cell lines consisted of up to 50% CD2+CD4-CD8- cells. None of the cell lines tested expressed surface immunoglobulin or other bovine B-cell markers. Thus, these long-term cell lines appear to include 2 T-lymphocyte subsets: the helper/inducer subset and a second subset expressing a phenotype similar to major histocompatibility complex-unrestricted cytolytic cells in other species.
通过用流产布鲁氏菌和丝裂霉素C处理的自体抗原呈递细胞反复刺激，建立了与流产布鲁氏菌反应的牛T细胞系。使用来自14头牛的33个细胞系获得了代表性结果。在胸腺嘧啶核苷掺入试验中，培养物对强毒实验室菌株2308、疫苗菌株19和粗糙突变菌株RB51有反应。这些培养物中的细胞对流产布鲁氏菌的反应需要抗原呈递细胞。自体抗原呈递细胞对大多数测试细胞系是最佳的，尽管一些T细胞系在存在某些但不是所有同种异体抗原呈递细胞的情况下可以对流产布鲁氏菌作出反应。这些细胞系表达了活化牛T细胞的细胞表面标志物特征。在测试簇决定簇（CD）4和CD8细胞表面抗原表达的细胞系中，任何培养物中的细胞均未表达牛CD8等同物，但所有培养物均含有数量不等的CD4+细胞。一些细胞系由高达50%的CD2+CD4-CD8-细胞组成。所测试的细胞系均未表达表面免疫球蛋白或其他牛B细胞标志物。因此，这些长期细胞系似乎包括2个T淋巴细胞亚群：辅助/诱导亚群和第二个表达与其他物种中主要组织相容性复合体非限制性溶细胞性细胞相似表型的亚群。
