Smith R, Kapatsa J C, Sherwood S J, Ficht T A, Templeton J W, Adams L G
Department of Veterinary Pathology, Texas A&M University, College Station 77843-4463.
Am J Vet Res. 1990 Apr;51(4):518-23.
The reactivity of bovine lymphocytes to 4 species of Brucella was tested in thymidine-uptake assays, using long-term cultured lymphocytes and freshly obtained blood mononuclear cells. Lymphocytes were taken from cows that had been challenge exposed with a virulent strain of B abortus at midgestation. The cows were classified retrospectively as being naturally resistant or susceptible to brucellosis. Lymphocytes taken from these cows had 3 patterns of reactivity with species of Brucella: pattern 1 was defined by reactivity with 4 species (B abortus, B canis, B suis, and B melitensis); pattern 2 was defined by reactivity with all these species, except B melitensis; pattern 3 was defined by reactivity with B abortus and B canis, but not with B suis or B melitensis. There was a statistically significant correlation between susceptibility to brucellosis and expression of lymphocyte cross-reactivity with B suis (P less than 0.01) and with B melitensis (P less than 0.001).
利用长期培养的淋巴细胞和新鲜获取的血液单核细胞，通过胸腺嘧啶核苷摄取试验检测了牛淋巴细胞对4种布鲁氏菌的反应性。淋巴细胞取自妊娠中期经强毒流产布鲁氏菌攻击暴露的奶牛。这些奶牛经回顾性分类为对布鲁氏菌病天然抗性或易感。取自这些奶牛的淋巴细胞对布鲁氏菌属有3种反应模式：模式1由对4种布鲁氏菌（流产布鲁氏菌、犬布鲁氏菌、猪布鲁氏菌和羊布鲁氏菌）的反应性定义；模式2由对除羊布鲁氏菌外的所有这些菌的反应性定义；模式3由对流产布鲁氏菌和犬布鲁氏菌的反应性定义，但对猪布鲁氏菌或羊布鲁氏菌无反应。布鲁氏菌病易感性与淋巴细胞对猪布鲁氏菌（P<0.01）和羊布鲁氏菌（P<0.001）交叉反应性表达之间存在统计学显著相关性。
