通过电穿孔对枯草芽孢杆菌完整细胞进行遗传转化。
Genetic transformation of intact cells of Bacillus subtilis by electroporation.
作者信息
Brigidi P, De Rossi E, Bertarini M L, Riccardi G, Matteuzzi D
机构信息
Department of Pharmaceutical Sciences, University of Bologna, Italy.
出版信息
FEMS Microbiol Lett. 1990 Jan 15;55(1-2):135-8. doi: 10.1016/0378-1097(90)90182-p.
Plasmid DNAs were introduced by electroporation into Bacillus subtilis PB1424 as an alternative to competent-cell or protoplast transformation. The maximum electroporation efficiency was 10(4) transformants/microgram DNA. Parameters including growth phase of cells, ionic strength of the suspending medium, concentration and size of plasmid DNAs, amplitude and duration of the pulse, were evaluated in order to determine conditions that improved transformation efficiency.
通过电穿孔将质粒DNA导入枯草芽孢杆菌PB1424，以此作为感受态细胞或原生质体转化的替代方法。最大电穿孔效率为每微克DNA产生10⁴个转化体。对包括细胞生长阶段、悬浮培养基的离子强度、质粒DNA的浓度和大小、脉冲幅度和持续时间等参数进行了评估，以确定提高转化效率的条件。
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