在枯草芽孢杆菌NB22（一种抗真菌抗生素伊枯草菌素的产生菌）中进行质粒转化。

Plasmid transformation in Bacillus subtilis NB22, an antifungal-antibiotic iturin producer.

作者信息

Matsuno Y, Hiraoka H, Ano T, Shoda M

机构信息

Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Yokohama, Japan.

出版信息

FEMS Microbiol Lett. 1990 Jan 15;55(1-2):227-9. doi: 10.1016/0378-1097(90)90200-a.

DOI:10.1016/0378-1097(90)90200-a

PMID:2109723

Abstract

A transformation system with plasmids was developed for Bacillus subtilis NB22, an antibiotic iturin producing strain. Treatment of B. subtilis NB22 with 4 M KCl was effective for the induction of competence, followed by uptake of plasmid DNA in the presence of polyethylene glycol. The efficiency of transformation of this bacterium with pC194 and pUB110 was 4.1 X 10(3) and 1.5 X 10(3) transformants per micrograms DNA, respectively and the transformation frequency was 3.3 X 10(-3) and 7.2 X 10(-4), transformants per viable cell, respectively. This method was much faster and three orders of magnitude more efficient in transformation efficiency than protoplast transformation methods.

摘要

为产抗真菌素iturin的枯草芽孢杆菌NB22构建了一个带有质粒的转化系统。用4M KCl处理枯草芽孢杆菌NB22可有效诱导其感受态，随后在聚乙二醇存在的情况下摄取质粒DNA。用pC194和pUB110转化该细菌的效率分别为每微克DNA产生4.1×10³和1.5×10³个转化子，转化频率分别为每存活细胞3.3×10⁻³和7.2×10⁻⁴个转化子。该方法比原生质体转化方法快得多，转化效率高三个数量级。