寡聚赖氨酸Lys-3、Lys-4和Lys-5对铜绿假单胞菌外膜通透性的影响。

The effect of oligolysines Lys-3, Lys-4, and Lys-5 on the outer membrane permeability of Pseudomonas aeruginosa.

作者信息

Vaara M

机构信息

Department of Microbiology and Immunology, University of California, Berkeley 94720.

出版信息

FEMS Microbiol Lett. 1990 Jan 15;55(1-2):15-9. doi: 10.1016/0378-1097(90)90160-r.

DOI:10.1016/0378-1097(90)90160-r

PMID:2109719

Abstract

A lysine polymer with five residues (Lys-5) was found to remarkably increase the outer membrane (OM) permeability of Pseudomonas aeruginosa to the tested hydrophobic probes (nitrocefin, N-phenyl naphthylamine, rifampin). Lys-3 and Lys-4 were inactive. The OM of Escherichia coli and Salmonella typhimurium was not permeabilized by Lys-5. Furthermore, even the action of Lys-5 on the Pseudomonas OM was abolished when the assays were performed in the presence of 150 mM NaCl instead of the low-ionic strength buffer earlier used by investigators studying the effect of polycations on the Pseudomonas OM.

摘要

研究发现，一种具有五个残基的赖氨酸聚合物（Lys-5）能显著提高铜绿假单胞菌外膜（OM）对所测试疏水性探针（头孢硝噻吩、N-苯基萘胺、利福平）的通透性。Lys-3和Lys-4没有活性。Lys-5不会使大肠杆菌和鼠伤寒沙门氏菌的外膜发生通透化。此外，当在150 mM NaCl存在的条件下进行测定时，Lys-5对铜绿假单胞菌外膜的作用消失了，而不是像之前研究聚阳离子对铜绿假单胞菌外膜影响的研究人员所使用的低离子强度缓冲液那样。