Latger-Cannard Véronique, Bardet Valérie, Malet Michèle, Lagrange Monique, Empereur Fabienne, Fenneteau Odile
University Teaching Hospital, Nancy, France.
Lab Hematol. 2010 Dec;16(4):76-82. doi: 10.1532/LH96.10007.
Cytochemical detection of myeloperoxidase (MPO) activity, a strong marker for myeloid differentiation, is usually performed by benzidine dihydrochloride staining, with the threshold at 3%. Several reports have demonstrated the potential toxicity of benzidine, and bans have been issued, under French law, prohibiting female technicians from being exposed to the aromatic hydrocarbon group, including benzidine. The aim of this study was to test an alpha-naphthol and pyronine-based substitute using a standardized kit (MYELOPEROXIDASE KIT, RAL [Réactifs RAL, Martillac, France]) to measure MPO activity in blast cells. This prospective, multicenter study made it possible to analyze 101 acute leukemia (AL) cases; it has also demonstrated both the 96% specificity and the 99% sensitivity of the method, with a threshold for positive staining of 3%, as well as good correlation (r = 0.95) between the staining method tested and the benzidine staining method. When using the alpha-naphthol/pyronine-based staining for MPO, the mean number of positive blast cells is statistically lower than that obtained using benzidine, but without incidence on AL classification. These results allow us to conclude that this method makes it possible to classify acute blood diseases by measuring MPO activity using reagents permitted by law, according to a standardized and reproducible protocol.
髓过氧化物酶（MPO）活性的细胞化学检测是髓系分化的一个重要标志物，通常通过盐酸联苯胺染色进行，阈值为3%。有几份报告证明了联苯胺的潜在毒性，根据法国法律已发布禁令，禁止女性技术人员接触包括联苯胺在内的芳烃类物质。本研究的目的是使用标准化试剂盒（MYELOPEROXIDASE KIT，RAL [Réactifs RAL，法国马蒂亚克]）测试一种基于α-萘酚和派洛宁的替代方法，以测量原始细胞中的MPO活性。这项前瞻性、多中心研究分析了101例急性白血病（AL）病例；它还证明了该方法的特异性为96%，敏感性为99%，阳性染色阈值为3%，以及所测试的染色方法与联苯胺染色方法之间具有良好的相关性（r = 0.95）。当使用基于α-萘酚/派洛宁的染色法检测MPO时，原始阳性细胞的平均数量在统计学上低于使用联苯胺时，但对AL的分类没有影响。这些结果使我们能够得出结论，该方法能够根据标准化且可重复的方案，使用法律允许的试剂通过测量MPO活性对急性血液疾病进行分类。
