Chen Wan-Xin, Zhu Hong-Lin, Xue Mei, Zhou Hao, Zhao Fei, Yan Ni, Chen Yan
Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Acta Histochem. 2014 Jan;116(1):292-6. doi: 10.1016/j.acthis.2013.08.001. Epub 2013 Sep 14.
Myeloperoxidase (MPO) staining has been important for the cytomorphological diagnosis and classification of leukemia. A novel staining method for MPO and its clinical application are presented in the report. Pyronine B (PyB), serving as a chromogenic reagent, was pre-oxidized to obtain stable oxidized Pyronine B solution. The MPO working solution for oxidized pyronine B method consisted of phosphate buffer solution, potassium iodide (KI) solution, and oxidized Pyronine B solution. The positive products of the oxidized Pyronine B method of MPO staining were vibrant red particles located in cytoplasm and the nucleus was stained bluish green. Bone marrow smears from 229 patients with acute leukemia or with grossly normal bone marrow were stained by both oxidized Pyronine B method and the conventional Washburn benzidine staining and a comparison revealed no significant difference in the positive detection rate between the two techniques. The new method eliminates the influence of the varying amount of H₂O₂ on MPO staining. With this method, the reagents were more stable and the staining procedure was simple and time-saving. This MPO staining technique is a better alternative than the conventional benzidine-based methods.
髓过氧化物酶（MPO）染色对于白血病的细胞形态学诊断和分类具有重要意义。本报告介绍了一种MPO的新型染色方法及其临床应用。以吡罗红B（PyB）作为显色剂，对其进行预氧化以获得稳定的氧化吡罗红B溶液。氧化吡罗红B法的MPO工作液由磷酸盐缓冲液、碘化钾（KI）溶液和氧化吡罗红B溶液组成。MPO染色氧化吡罗红B法的阳性产物为位于细胞质中的鲜红色颗粒，细胞核染成蓝绿色。对229例急性白血病患者或骨髓大致正常的患者的骨髓涂片采用氧化吡罗红B法和传统的沃什伯恩联苯胺染色法进行染色，比较发现两种技术的阳性检出率无显著差异。新方法消除了不同量的H₂O₂对MPO染色的影响。采用该方法，试剂更稳定，染色过程简单且省时。这种MPO染色技术是比传统基于联苯胺的方法更好的选择。
