Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.
Plant Cell Physiol. 2011 Jan;52(1):205-9. doi: 10.1093/pcp/pcq181. Epub 2010 Nov 19.
While exogenous toxic compounds such as herbicides are thought to be sequestered into vacuoles in the form of glutathione (GSH) conjugates, little is understood about natural plant products conjugated with GSH. To identify natural products conjugated with GSH in plants, metabolites in the Arabidopsis γ-glutamyl transpeptidase (ggt) 4 knockout mutants that are blocked in the degradation of GSH conjugates in the vacuole were compared with those in wild-type plants. Among the metabolites identified, one was confirmed to be the 12-oxo-phytodienoic acid (OPDA)-GSH conjugate, indicating that OPDA, a precursor of jasmonic acid (JA), is transported into the vacuole as a GSH conjugate.
虽然人们认为外源性有毒化合物如除草剂以谷胱甘肽 (GSH) 缀合物的形式被隔离到液泡中，但对于与 GSH 缀合的天然植物产物知之甚少。为了鉴定植物中与 GSH 缀合的天然产物，比较了在 Arabidopsis γ-谷氨酰转肽酶 (ggt) 4 敲除突变体中不能降解液泡中 GSH 缀合物的代谢物与野生型植物中的代谢物。在所鉴定的代谢物中，有一种被确认为 12-氧-植保素酸 (OPDA)-GSH 缀合物，表明茉莉酸 (JA) 的前体 OPDA 作为 GSH 缀合物被运输到液泡中。
