向群体感应信号 AI-2 的趋化作用需要 Tsr 化学感受器和周质 LsrB AI-2 结合蛋白。
Chemotaxis to the quorum-sensing signal AI-2 requires the Tsr chemoreceptor and the periplasmic LsrB AI-2-binding protein.
机构信息
3122 TAMU, Texas A&M University, College Station, TX 77843-3122, USA.
出版信息
J Bacteriol. 2011 Feb;193(3):768-73. doi: 10.1128/JB.01196-10. Epub 2010 Nov 19.
Abstract
AI-2 is an autoinducer made by many bacteria. LsrB binds AI-2 in the periplasm, and Tsr is the l-serine chemoreceptor. We show that AI-2 strongly attracts Escherichia coli. Both LsrB and Tsr are necessary for sensing AI-2, but AI-2 uptake is not, suggesting that LsrB and Tsr interact directly in the periplasm.
摘要
AI-2 是许多细菌产生的一种自体诱导物。LsrB 在周质空间中结合 AI-2，而 Tsr 是 l-丝氨酸化学感受器。我们发现 AI-2 能强烈吸引大肠杆菌。LsrB 和 Tsr 都是感应 AI-2 所必需的，但 AI-2 的摄取并非必需，这表明 LsrB 和 Tsr 在周质空间中直接相互作用。
相似文献
1
Chemotaxis to the quorum-sensing signal AI-2 requires the Tsr chemoreceptor and the periplasmic LsrB AI-2-binding protein.
J Bacteriol. 2011 Feb;193(3):768-73. doi: 10.1128/JB.01196-10. Epub 2010 Nov 19.
2
Identification of novel autoinducer-2 receptors in reveals plasticity in the binding site of the LsrB receptor family.
J Biol Chem. 2019 Mar 22;294(12):4450-4463. doi: 10.1074/jbc.RA118.006938. Epub 2019 Jan 29.
3
Sensing of autoinducer-2 by functionally distinct receptors in prokaryotes.
Nat Commun. 2020 Oct 23;11(1):5371. doi: 10.1038/s41467-020-19243-5.
4
Quorum sensing in thermophiles: prevalence of autoinducer-2 system.
BMC Microbiol. 2018 Jun 28;18(1):62. doi: 10.1186/s12866-018-1204-x.
5
Lsr operon is associated with AI-2 transfer and pathogenicity in avian pathogenic Escherichia coli.
Vet Res. 2019 Dec 12;50(1):109. doi: 10.1186/s13567-019-0725-0.
6
Synthesis of d-desthiobiotin-AI-2 as a novel chemical probe for autoinducer-2 quorum sensing receptors.
Bioorg Chem. 2019 Nov;92:103200. doi: 10.1016/j.bioorg.2019.103200. Epub 2019 Aug 16.
7
LsrF, a coenzyme A-dependent thiolase, catalyzes the terminal step in processing the quorum sensing signal autoinducer-2.
Proc Natl Acad Sci U S A. 2014 Sep 30;111(39):14235-40. doi: 10.1073/pnas.1408691111. Epub 2014 Sep 15.
8
Thiophenone Attenuates Enteropathogenic Escherichia coli O103:H2 Virulence by Interfering with AI-2 Signaling.
PLoS One. 2016 Jun 16;11(6):e0157334. doi: 10.1371/journal.pone.0157334. eCollection 2016.
9
Chemorepulsion from the Quorum Signal Autoinducer-2 Promotes Helicobacter pylori Biofilm Dispersal.
mBio. 2015 Jul 7;6(4):e00379. doi: 10.1128/mBio.00379-15.
10
Differential gene expression shows natural brominated furanones interfere with the autoinducer-2 bacterial signaling system of Escherichia coli.
Biotechnol Bioeng. 2004 Dec 5;88(5):630-42. doi: 10.1002/bit.20259.
引用本文的文献
1
Navigating bacterial motility through chemotaxis: from molecular mechanisms to physiological perspectives.
Folia Microbiol (Praha). 2025 Aug 9. doi: 10.1007/s12223-025-01301-4.
2
Interplay between chemotaxis, quorum sensing, and metabolism regulates Escherichia coli-Salmonella Typhimurium interactions in vivo.
PLoS Pathog. 2025 May 2;21(5):e1013156. doi: 10.1371/journal.ppat.1013156. eCollection 2025 May.
3
Combined Analysis of Transcriptomes and Metabolomes Reveals Key Genes and Substances That Affect the Formation of a Multi-Species Biofilm by Nine Gut Bacteria.
Microorganisms. 2025 Jan 22;13(2):234. doi: 10.3390/microorganisms13020234.
4
Spatial structure, chemotaxis and quorum sensing shape bacterial biomass accumulation in complex porous media.
Nat Commun. 2024 Jan 2;15(1):191. doi: 10.1038/s41467-023-44267-y.
5
Chemical communication and its role in sexual selection across Animalia.
Commun Biol. 2023 Nov 20;6(1):1178. doi: 10.1038/s42003-023-05572-w.
6
Bacterial bioconvection confers context-dependent growth benefits and is robust under varying metabolic and genetic conditions.
J Bacteriol. 2023 Oct 26;205(10):e0023223. doi: 10.1128/jb.00232-23. Epub 2023 Oct 3.
7
Sensing preferences for prokaryotic solute binding protein families.
Microb Biotechnol. 2023 Sep;16(9):1823-1833. doi: 10.1111/1751-7915.14292. Epub 2023 Aug 7.
8
Conditions Necessary for the Transfer of Antimicrobial Resistance in Poultry Litter.
Antibiotics (Basel). 2023 Jun 3;12(6):1006. doi: 10.3390/antibiotics12061006.
9
CryoEM structure of a post-assembly MS-ring reveals plasticity in stoichiometry and conformation.
PLoS One. 2023 May 19;18(5):e0285343. doi: 10.1371/journal.pone.0285343. eCollection 2023.
10
Discovery of a New Chemoeffector for Chemoreceptor Tsr and Identification of a Molecular Mechanism of Repellent Sensing.
ACS Bio Med Chem Au. 2022 Mar 18;2(4):386-394. doi: 10.1021/acsbiomedchemau.1c00055. eCollection 2022 Aug 17.
本文引用的文献
1
Metabolic engineering to enhance bacterial hydrogen production.
Microb Biotechnol. 2008 Jan;1(1):30-9. doi: 10.1111/j.1751-7915.2007.00003.x.
2
Differences in signalling by directly and indirectly binding ligands in bacterial chemotaxis.
EMBO J. 2010 Oct 20;29(20):3484-95. doi: 10.1038/emboj.2010.224. Epub 2010 Sep 10.
3
Microfluidic techniques for the analysis of bacterial chemotaxis.
Methods Mol Biol. 2009;571:1-23. doi: 10.1007/978-1-60761-198-1_1.
4
Flow-based microfluidic device for quantifying bacterial chemotaxis in stable, competing gradients.
Appl Environ Microbiol. 2009 Jul;75(13):4557-64. doi: 10.1128/AEM.02952-08. Epub 2009 May 1.
5
Temporal regulation of enterohemorrhagic Escherichia coli virulence mediated by autoinducer-2.
Appl Microbiol Biotechnol. 2008 Apr;78(5):811-9. doi: 10.1007/s00253-008-1359-8. Epub 2008 Feb 7.
6
A LuxP-based fluorescent sensor for bacterial autoinducer II.
ACS Chem Biol. 2008 Feb 15;3(2):110-9. doi: 10.1021/cb7002048. Epub 2008 Jan 24.
7
Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research.
DNA Res. 2005;12(5):291-9. doi: 10.1093/dnares/dsi012. Epub 2006 Jan 9.
8
Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection.
Mol Syst Biol. 2006;2:2006.0008. doi: 10.1038/msb4100050. Epub 2006 Feb 21.
9
YdgG (TqsA) controls biofilm formation in Escherichia coli K-12 through autoinducer 2 transport.
J Bacteriol. 2006 Jan;188(2):587-98. doi: 10.1128/JB.188.2.587-598.2006.
10
Autoinducer 2 controls biofilm formation in Escherichia coli through a novel motility quorum-sensing regulator (MqsR, B3022).
J Bacteriol. 2006 Jan;188(1):305-16. doi: 10.1128/JB.188.1.305-316.2006.
Zotero 插件