Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República, 2780-157 Oeiras, Portugal.
Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República, 2780-157 Oeiras, Portugal; Instituto Gulbenkian da Ciência, 2781-901 Oeiras, Portugal.
Bioorg Chem. 2019 Nov;92:103200. doi: 10.1016/j.bioorg.2019.103200. Epub 2019 Aug 16.
In processes regulated by quorum sensing (QS) bacteria respond to the concentration of autoinducers in the environment to engage in group behaviours. Autoinducer-2 (AI-2) is unique as it can foster interspecies communication. Currently, two AI-2 receptors are known, LuxP and LsrB, but bacteria lacking these receptors can also respond to AI-2. In this work, we present an efficient and reproducible synthesis of a novel chemical probe, d-desthiobiotin-AI-2. This probe binds both LuxP and LsrB receptors from different species of bacteria. Thus, this probe is able to bind receptors that recognise the two known biologically active forms of AI-2, presenting the plasticity essential for the identification of novel unknown AI-2 receptors. Moreover, a protocol to pull down receptors bound to d-desthiobiotin-AI-2 with anti-biotin antibodies has also been established. Altogether, this work highlights the potential of conjugating chemical signals to biotinylated derivatives to identify and tag signal receptors involved in quorum sensing or other chemical signalling processes.
在群体感应（QS）调节的过程中，细菌会根据环境中自体诱导物的浓度来进行群体行为。自体诱导物-2（AI-2）是独特的，因为它可以促进种间通讯。目前，已知有两种 AI-2 受体，即 LuxP 和 LsrB，但缺乏这些受体的细菌也可以对 AI-2 做出反应。在这项工作中，我们提出了一种新的化学探针 d-去硫生物素-AI-2 的有效且可重复的合成方法。该探针可以结合来自不同种细菌的 LuxP 和 LsrB 受体。因此，该探针能够结合识别两种已知具有生物活性形式的 AI-2 的受体，从而提供了识别新的未知 AI-2 受体所必需的可塑性。此外，还建立了用抗生物素抗体下拉与 d-去硫生物素-AI-2 结合的受体的方案。总之，这项工作强调了将化学信号与生物素化衍生物结合起来识别和标记参与群体感应或其他化学信号转导过程的信号受体的潜力。
