Environmental and Molecular Microbiology Lab., Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Ralph M. Parsons Lab., Department of Civil and Environmental Engineering, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA.
Int J Syst Evol Microbiol. 2011 Oct;61(Pt 10):2464-2468. doi: 10.1099/ijs.0.024307-0. Epub 2010 Nov 19.
A yellow-pigmented, Gram-negative, short rod-shaped, non-motile and non-spore-forming bacterial strain, designated HU1-AH51(T), was isolated from freshwater sediment and was characterized using a polyphasic approach, in order to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity, strain HU1-AH51(T) was shown to belong to the genus Novosphingobium, showing the highest level of sequence similarity with respect to Novosphingobium resinovorum NCIMB 8767(T) (96.0 %), Novosphingobium naphthalenivorans TUT562(T) (96.0 %) and Novosphingobium panipatense SM16(T) (96.0 %). Strain HU1-AH51(T) had a genomic DNA G+C content of 62.6 mol% and Q-10 as the predominant respiratory quinone. Furthermore, the major polyamine component (spermidine) in the cytoplasm and the presence of sphingoglycolipids suggested that strain HU1-AH51(T) belongs to the family Sphingomonadaceae. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain HU1-AH51(T) represents a novel species of the genus Novosphingobium, for which the name Novosphingobium sediminicola sp. nov. is proposed. The type strain is HU1-AH51(T) ( = LMG 24320(T) = KCTC 22311(T)).
一株黄色、革兰氏阴性、短杆状、非运动和非孢子形成的细菌菌株，命名为 HU1-AH51(T)，从淡水沉积物中分离出来，并通过多相方法进行了表征，以确定其分类地位。根据 16S rRNA 基因序列相似性，菌株 HU1-AH51(T)属于新鞘氨醇单胞菌属，与新鞘氨醇单胞菌 NCIMB 8767(T)（96.0%）、萘烷单胞菌 TUT562(T)（96.0%）和泛菌 SM16(T)（96.0%）的序列相似度最高。菌株 HU1-AH51(T)的基因组 DNA G+C 含量为 62.6 mol%，Q-10 为主要呼吸醌。此外，细胞质中主要聚胺成分（亚精胺）和神经酰胺糖脂的存在表明，菌株 HU1-AH51(T)属于鞘氨醇单胞菌科。基于其表型特性和系统发育上的独特性，菌株 HU1-AH51(T)代表了新鞘氨醇单胞菌属的一个新种，现命名为 sediminicola 新种。模式菌株为 HU1-AH51(T)（=LMG 24320(T)=KCTC 22311(T)）。
