Botros S S
Pharmacology Department, Theodor Bilharz Research Institute, Imbaba, Guiza, Egypt.
Pharmacol Res. 1990 Mar-Apr;22(2):219-29. doi: 10.1016/1043-6618(90)90718-s.
Cytophotometric measurement of the effect of praziquantel (500 mg/kg for 2 days) versus hycanthone (60 mg/kg for 3 days) on hepatocyte nuclear deoxyribonucleic acid (DNA) content was evaluated in Schistosoma mansoni infected mice. Drugs were given 8 weeks post-infection and repeated weekly for 4 weeks. DNA values of infected untreated control and infected drug treated groups were related to the median and upper diploid DNA values of normal control. Schistosoma mansoni infection per se did not change the hepatocyte DNA content, yet aneuploidy was 16.7%. Praziquantel did not result in significant change of DNA content or ploidy, while hycanthone resulted in marked significant increase of DNA content (328.9%) and aneuploidy (100%), compared to infected untreated control. Histopathological examination revealed hyperchromatic nuclei with mitosis in the hepatocytes of hycanthone treated mice, but not in praziquantel treated animals. These DNA changes were found to correlate with the reported safety of praziquantel and the carcinogenicity of hycanthone.
在感染曼氏血吸虫的小鼠中，评估了吡喹酮（500毫克/千克，连用2天）与海恩酮（60毫克/千克，连用3天）对肝细胞核脱氧核糖核酸（DNA）含量的影响，并进行了细胞光度测定。在感染后8周给予药物，每周重复给药，共4周。将感染未治疗对照组和感染药物治疗组的DNA值与正常对照组的中位和上二倍体DNA值相关联。曼氏血吸虫感染本身并未改变肝细胞DNA含量，但非整倍体率为16.7%。与感染未治疗对照组相比，吡喹酮未导致DNA含量或倍性的显著变化，而海恩酮导致DNA含量显著增加（328.9%）和非整倍体率显著增加（100%）。组织病理学检查显示，海恩酮治疗小鼠的肝细胞中有核染色质过深且有有丝分裂现象，而吡喹酮治疗的动物则没有。发现这些DNA变化与报道的吡喹酮安全性和海恩酮致癌性相关。
