Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
Jpn J Infect Dis. 2010 Nov;63(6):401-4.
A laboratory colony of the mosquito Aedes japonicus japonicus, which has recently invaded the United States and is recognized as a highly competent vector of West Nile virus, was established from larvae collected in Narita, Japan. The mosquitoes were maintained with induced insemination, blood-feeding on humans, and oviposition in water provided from the original collection site during the first few generations, then the colony was transferred to a large cage (40×40×100 cm in height) and adapted to conditions in which specimens were allowed to mate freely. White mice were provided as the blood source, and deionized water was available for oviposition. Approximately 185 eggs, most of which were tolerant to desiccation for at least 1 month, with some surviving for up to 2.5 months, were obtained per female following a single blood-feeding. The rate of successful emergence was nearly 90%, although this rate decreased significantly at high larval densities. The colony has been maintained for 5 years, and developmental profiles of the species have been obtained during that time.
从日本成田收集的幼虫中建立了一个实验室群体的日本伊蚊（Aedes japonicus japonicus），该蚊最近已入侵美国，并被认为是西尼罗河病毒的高效传播媒介。在最初的几代中，通过诱导交配、以人类为血源和在从原始收集地提供的水中产卵来维持这些蚊子，然后将该蚊群转移到一个大型笼子（高 40×40×100 厘米）中，并适应于让标本自由交配的条件。用小白鼠作为血源，并提供去离子水用于产卵。雌性蚊子在单次吸血后可产下约 185 枚卵，其中大多数至少耐受干燥 1 个月，有些可存活长达 2.5 个月。尽管在高幼虫密度下，成功孵化的比率显著降低，但孵化率接近 90%。该蚊群已维持了 5 年，在此期间获得了该物种的发育概况。
