Department of Dermatology, Mie University Graduate School of Medicine, Japan.
Allergol Int. 2011 Mar;60(1):45-51. doi: 10.2332/allergolint.10-OA-0198. Epub 2010 Nov 24.
Allergen-specific immunotherapy (SIT) is currently used for several allergic disorders and IL-10-producing regulatory T cells (Tr1) induced by SIT suppress allergic reactions. We investigated the relation between IL-10 production and acquiring allergy.
A prospective study was undertaken to evaluate the effect of SIT on IL-10 production in T cells and other cell fractions in children with pollinosis. In addition, blood samples were collected from non-allergic healthy controls and patients with pollinosis to compare the levels of IL-10 production. PBMC were cultured with pollen peptides or control allergens, and the IL-10 production from monocyte and CD4 T cell was analyzed.
Monocytes and CD4 T cells from SIT group of patients produced high levels of IL-10, suggesting that the induction of IL-10 is essential for inducing T cell tolerance. IL-10 production from monocytes and T cells was significantly increased in non-allergic controls compared to patients with pollinosis. This high IL-10 production was observed even when PBMC were stimulated with antigens other than pollen peptides.
IL-10 is critical for induction of specific T cell tolerance, and increased production of IL-10 by monocytes and T cells during inflammatory responses or after SIT may influence effector cells in allergy. Present data implicates that the low productivity of IL-10 by monocytes and T cells is closely related with sensitivity to multiple allergens, and resistance to allergic diseases. Augmentation of constitutive IL-10 production from immune system is a potential therapeutic approach for allergic disorders.
变应原特异性免疫疗法（SIT）目前用于多种过敏性疾病，SIT 诱导的 IL-10 产生的调节性 T 细胞（Tr1）可抑制过敏反应。我们研究了 SIT 诱导的 IL-10 产生与获得过敏之间的关系。
进行了一项前瞻性研究，以评估 SIT 对花粉症儿童 T 细胞和其他细胞群中 IL-10 产生的影响。此外，还从非过敏性健康对照者和花粉症患者中采集血样，以比较 IL-10 产生水平。用花粉肽或对照变应原培养 PBMC，并分析单核细胞和 CD4 T 细胞的 IL-10 产生。
来自 SIT 组患者的单核细胞和 CD4 T 细胞产生高水平的 IL-10，表明 IL-10 的诱导对于诱导 T 细胞耐受至关重要。与花粉症患者相比，非过敏性对照者的单核细胞和 T 细胞产生的 IL-10 明显增加。即使在用抗原而非花粉肽刺激 PBMC 时，也观察到这种高 IL-10 产生。
IL-10 对于诱导特异性 T 细胞耐受至关重要，在炎症反应或 SIT 后，单核细胞和 T 细胞中 IL-10 的产生增加可能会影响过敏中的效应细胞。目前的数据表明，单核细胞和 T 细胞中 IL-10 的低产量与对多种变应原的敏感性以及对过敏性疾病的抵抗力密切相关。增强免疫系统中 IL-10 的组成性产生可能是治疗过敏疾病的一种潜在治疗方法。
