Dermatology and Ophthalmology Research, Institute for Regenerative Cures, University of California Davis School of Medicine, Sacramento, CA 95817, USA.
Cornea. 2011 Mar;30(3):338-43. doi: 10.1097/ICO.0b013e3181f7f2de.
To measure electric current in human corneal wounds and test the feasibility of pharmacologically enhancing the current to promote corneal wound healing.
Using a noninvasive vibrating probe, corneal electric current was measured before and after wounding of the epithelium of donated postmortem human corneas. The effects of drug aminophylline and chloride-free solution on wound current were also tested.
Unwounded cornea had small outward currents (0.07 μA/cm²). Wounding increased the current more than 5 fold (0.41 μA/cm²). Monitoring the wound current over time showed that it seemed to be actively regulated and maintained above normal unwounded levels for at least 6 hours. The time course was similar to that previously measured in rat cornea. Drug treatment or chloride-free solution more than doubled the size of wound currents.
Electric current at human corneal wounds can be significantly increased with aminophylline or chloride-free solution. Because corneal wound current directly correlates with wound healing rate, our results suggest a role for chloride-free and/or aminophylline eyedrops to enhance healing of damaged cornea in patients with reduced wound healing such as the elderly or diabetic patient. This novel approach offers bioelectric stimulation without electrodes and can be readily tested in patients.
测量人眼角膜伤口的电流，并测试通过药理学增强电流来促进角膜伤口愈合的可行性。
使用非侵入性振动探针，测量死后捐献眼角膜上皮受伤前后的角膜电流。还测试了药物氨茶碱和无氯溶液对伤口电流的影响。
未受伤的角膜有微弱的外向电流（0.07 μA/cm²）。受伤后电流增加了 5 倍以上（0.41 μA/cm²）。随着时间的推移监测伤口电流表明，它似乎被主动调节并维持在正常未受伤水平以上至少 6 小时。其时间过程与之前在大鼠角膜中测量的相似。药物治疗或无氯溶液使伤口电流增加了一倍以上。
可以通过氨茶碱或无氯溶液显著增加人眼角膜伤口的电流。由于角膜伤口电流与伤口愈合速度直接相关，我们的结果表明，无氯和/或氨茶碱滴眼剂可用于增强愈合受损角膜的作用，特别是在愈合能力降低的患者中，如老年人或糖尿病患者。这种新方法提供了无需电极的生物电刺激，并且可以在患者中进行快速测试。
