ARC Centre of Excellence for Free Radical Chemistry and Biotechnology, Research School of Chemistry, Australian National University, Canberra ACT 0200, Australia.
Chem Commun (Camb). 2011 Feb 14;47(6):1839-41. doi: 10.1039/c0cc02879g. Epub 2010 Nov 22.
3-Chloro-Abu and 4-chloro-Nva are biosynthetically incorporated into E. coli peptidyl-Pro cis-trans isomerase B, as substitutes for Val and Leu, respectively. The extent of incorporation is up to ~90%, and substituted protein is catalytically active. By contrast, 4-chloro-Val is not an effective replacement for Ile.
3-氯-Abu 和 4-氯-Nva 分别作为 Val 和 Leu 的类似物，被生物合成掺入到大肠杆菌肽酰脯氨顺反异构酶 B 中。掺入程度高达约 90%，且取代蛋白具有催化活性。相比之下，4-氯-Val 不能有效地替代 Ile。
