将脂肪族含酮氨基酸遗传掺入蛋白质中用于其定点修饰。
Genetic incorporation of an aliphatic keto-containing amino acid into proteins for their site-specific modifications.
机构信息
Department of Chemistry, Texas A&M University, College Station, TX 77843, United States.
出版信息
Bioorg Med Chem Lett. 2010 Feb 1;20(3):878-80. doi: 10.1016/j.bmcl.2009.12.077. Epub 2009 Dec 24.
PMID:20074948
Abstract
2-Amino-8-oxononanoic acid has been genetically incorporated into proteins in Escherichia coli by the use of an evolved pyrrolysyl-tRNA synthetase/pylT pair. The direct usage of the exclusive reactivity of the keto group in this amino acid with hydrazide- and alkoxyamine-bearing compounds to site-specifically label proteins under a mild condition close to physiological pH exhibited a very high efficiency.
摘要
2-氨基-8-氧代壬酸已通过使用进化的吡咯赖氨酸 tRNA 合成酶/ pyrT 对在大肠杆菌中掺入蛋白质。在接近生理 pH 的温和条件下，该氨基酸中酮基的独特反应性与带有酰腙和烷氧基胺的化合物直接反应，以高效、高特异性地标记蛋白质。
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