State Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, 138 Yixueyuan Road, Shanghai, 200032, People's Republic of China.
Mol Biol Rep. 2011 Apr;38(4):2557-62. doi: 10.1007/s11033-010-0394-6. Epub 2010 Nov 21.
Regulated endocrine-specific protein-18 (RESP18) is distributed mainly in the peripheral endocrine and neuroendocrine tissues. The expression of RESP18 protein is regulated by physiological factors, such as blood glucose or dopaminergic drugs, but its functions remain unclear. In this study, to explore the biological functions of RESP18 in vivo, we generated RESP18 heterozygous deficient mice, and further found RESP18 was essential for embryonic development. In addition, we cloned a new isoform of mouse RESP18 by reverse transcription-polymerase chain reaction (RT-PCR), and denominated it as RESP18-c. Mouse RESP18-c, by skipping exon4 (43 bp in length), encodes a shorter protein of 120 amino acid residues. The distribution of RESP18-c mRNA is similar with that of RESP18 mRNA in the peripheral tissues and brains of mice.
受调控的内分泌特异性蛋白 18（RESP18）主要分布在外周内分泌和神经内分泌组织中。RESP18 蛋白的表达受生理因素（如血糖或多巴胺能药物）的调节，但功能尚不清楚。在这项研究中，为了探索 RESP18 在体内的生物学功能，我们生成了 RESP18 杂合缺失小鼠，并进一步发现 RESP18 对胚胎发育是必需的。此外，我们通过反转录-聚合酶链反应（RT-PCR）克隆了小鼠 RESP18 的一种新的异构体，并将其命名为 RESP18-c。小鼠 RESP18-c 通过跳过外显子 4（43 个碱基长），编码一个 120 个氨基酸残基的较短蛋白质。RESP18-c mRNA 在小鼠外周组织和大脑中的分布与 RESP18 mRNA 相似。
