Department of Physics and Astronomy, National Center for Creative Research Initiatives, Seoul National University, Seoul 151-747, Korea.
Angew Chem Int Ed Engl. 2010 Dec 17;49(51):9922-5. doi: 10.1002/anie.201005402.
We developed a single-molecule four-color FRET technique both in confocal and in total-internal-reflection fluorescence microscopies. Real-time determination of six inter-fluorophore FRET efficiencies allowed us to probe the correlated motion of four arms of the Holliday junction. The technique was also applied to assess the correlation of RecA-mediated strand exchange events at both ends of a synaptic complex.
我们开发了一种单分子四色 FRET 技术,可用于共聚焦和全内反射荧光显微镜。实时测定六个荧光团之间的 FRET 效率,使我们能够探测 Holliday 连接体四个臂的相关运动。该技术还用于评估突触复合物两端 RecA 介导的链交换事件的相关性。