Department of Biophysics and Chemical Biology, Seoul National University, Seoul 151-747, Korea.
Chem Commun (Camb). 2010 Jul 14;46(26):4683-5. doi: 10.1039/c002666b. Epub 2010 Jun 1.
Using a single-molecule fluorescence method uniquely suitable for binding assay, alternating-laser excitation fluorescence resonance energy transfer (ALEX-FRET), we accurately measured the cleavage rate of 8-17 deoxyribozyme, an RNA-cleaving enzyme, at the single-molecule level in real time with a minimum consumption of samples, i.e., at least three orders of magnitude smaller than used in the conventional ensemble FRET method.
我们使用一种独特适用于结合分析的单分子荧光方法——交替激光激发荧光共振能量转移（ALEX-FRET），以最小的样品消耗（即比传统的集合 FRET 方法小至少三个数量级），实时、准确地在单分子水平上测量了 8-17 脱氧核酶（一种 RNA 切割酶）的切割速率。
