Edwards Lindsay M, Murray Andrew J, Holloway Cameron J, Carter Emma E, Kemp Graham J, Codreanu Ion, Brooker Helen, Tyler Damian J, Robbins Peter A, Clarke Kieran
Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, UK.
FASEB J. 2011 Mar;25(3):1088-96. doi: 10.1096/fj.10-171983. Epub 2010 Nov 24.
We recently showed that a short-term high-fat diet blunted exercise performance in rats, accompanied by increased uncoupling protein levels and greater respiratory uncoupling. In this study, we investigated the effects of a similar diet on physical and cognitive performance in humans. Twenty sedentary men were assessed when consuming a standardized, nutritionally balanced diet (control) and after 7 d of consuming a diet comprising 74% kcal from fat. Efficiency was measured during a standardized exercise task, and cognition was assessed using a computerized assessment battery. Skeletal muscle mitochondrial function was measured using (31)P magnetic resonance spectroscopy. The diet increased mean ± se plasma free fatty acids by 44% (0.32±0.03 vs. 0.46±0.05 mM; P<0.05) and decreased whole-body efficiency by 3% (21±1 vs. 18±1%; P<0.05), although muscle uncoupling protein (UCP3) content and maximal mitochondrial function were unchanged. High-fat diet consumption also increased subjects' simple reaction times (P<0.01) and decreased power of attention (P<0.01). Thus, we have shown that a high-fat diet blunts whole-body efficiency and cognition in sedentary men. We suggest that this effect may be due to increased respiratory uncoupling.
我们最近发现，短期高脂饮食会削弱大鼠的运动表现，同时伴随着解偶联蛋白水平的升高和更强的呼吸解偶联。在本研究中，我们调查了类似饮食对人类身体和认知表现的影响。对20名久坐不动的男性在食用标准化、营养均衡的饮食（对照组）时以及在食用由74%千卡脂肪组成的饮食7天后进行了评估。在一项标准化运动任务中测量效率，并使用计算机化评估组合来评估认知。使用（31）P磁共振波谱测量骨骼肌线粒体功能。该饮食使平均±标准误血浆游离脂肪酸增加了44%（0.32±0.03对0.46±0.05 mM；P<0.05），并使全身效率降低了3%（21±1对18±1%；P<0.05），尽管肌肉解偶联蛋白（UCP3）含量和最大线粒体功能没有变化。食用高脂饮食还增加了受试者的简单反应时间（P<0.01）并降低了注意力集中度（P<0.01）。因此，我们已经表明高脂饮食会削弱久坐男性的全身效率和认知能力。我们认为这种影响可能是由于呼吸解偶联增加所致。
