Takahashi H, Parsons P G, Favier D, McEwan M, Strutton G M, Akutsu Y, Jimbow K
Queensland Institute of Medical Research, Herston, Australia.
Virchows Arch A Pathol Anat Histopathol. 1990;416(6):513-9. doi: 10.1007/BF01600302.
We have generated monoclonal antibodies (MoAbs) against melanosomal proteins (MoAb 1C11 and MoAb HMSA-1) and a cytoplasmic protein strongly synthesized in neoplastic melanocytes but not associated with melanogenesis (MoAb 7H11). An immunohistochemical study of paraffin sections showed that nearly 90% of epidermal neoplastic melanocytes, including melanomas, expressed 1C11 antigen, whereas this antigen was poorly preserved in dermal melanocytic cells except melanomas. HMSA-1 antigen was expressed in a complementary manner to 1C11 antigen, being found in dermal naevus cells but not generally in the epidermal regions, except for dysplastic naevi and melanomas. In contrast, 7H11 antigen was distributed in nearly 90% of melanocytic tumours except solar lentigo and lentigo maligna lesions. The failure of MoAb 1C11 to react with dermal melanocytes may reflect a subtle alteration in melanogenesis during tumour evolution. Overall, the combined use of MoAbs serves as an accurate diagnosis of melanocytic tumours, the pigment-independent MoAb 7H11 being particularly useful for amelanotic and metastatic lesions.
我们已经制备了针对黑素小体蛋白的单克隆抗体（MoAb 1C11和MoAb HMSA-1）以及一种在肿瘤性黑素细胞中强烈合成但与黑素生成无关的细胞质蛋白（MoAb 7H11）。对石蜡切片的免疫组织化学研究表明，包括黑色素瘤在内的近90%的表皮肿瘤性黑素细胞表达1C11抗原，而除黑色素瘤外，该抗原在真皮黑素细胞中保存较差。HMSA-1抗原以与1C11抗原互补的方式表达，见于真皮痣细胞，但除发育异常痣和黑色素瘤外，一般不在表皮区域表达。相比之下，7H11抗原分布于除日光性雀斑样痣和恶性雀斑样痣病变外的近90%的黑素细胞肿瘤中。MoAb 1C11不能与真皮黑素细胞反应可能反映了肿瘤演变过程中黑素生成的细微改变。总体而言，单克隆抗体的联合使用有助于黑素细胞肿瘤的准确诊断，与色素无关的MoAb 7H11对无色素性和转移性病变特别有用。
