Department of Food and Biotechnology, Hanseo University, Seosan, Chungnam, South Korea.
Menopause. 2011 Mar;18(3):307-13. doi: 10.1097/gme.0b013e3181f31b1f.
Osteoporosis is a major health problem worldwide, and most current therapy used in osteoporosis treatment acts by either increasing bone formation or decreasing bone resorption. However, the adverse effects of these therapies may preclude their long-term use. We examined the effects of egg yolk water-soluble peptide (YPEP) on bone metabolism as an alternative to current therapeutic agents in ovariectomized (OVX) rats.
In the first step, the in vitro effects of YPEP on bone loss were determined. The proliferation, collagen content, and alkaline phosphatase activity of preosteoblastic MC3T3-E1 cells and osteoclastogenesis from bone marrow-derived precursor cells were measured. The in vivo experiment confirmed the positive effect of YPEP on bone tissue. Three-month-old female Sprague-Dawley rats were either sham operated or ovariectomized and fed commercial chow diet or 0.1% YPEP-supplemented diet for 3 month.
YPEP increased preosteoblastic MC3T3-E1 cell proliferation and alkaline phosphatase activity in a dose-dependent manner. Collagen content was also increased by YPEP treatment. Furthermore, YPEP potently suppressed osteoclastogenesis from bone marrow-derived precursor cells. YPEP (100 μg/mL) abolished the formation of osteoclasts positive for tartrate-resistant acid phosphatase. OVX rats supplemented with YPEP showed an osteoprotective effect, as the bone mineral density and cortical thickness in the tibia were increased compared with the OVX controls. Moreover, histological data indicate that YPEP prevented the cancellous bone loss induced by ovariectomy. None of these protective effects were observed in casein-treated rats.
The present study suggests that YPEP is a promising alternative to current therapeutic agents for the management of osteoporosis.
骨质疏松症是全球范围内的一个主要健康问题，目前大多数用于骨质疏松症治疗的疗法要么通过增加骨形成，要么通过减少骨吸收来发挥作用。然而，这些治疗方法的不良反应可能会限制其长期使用。我们研究了蛋黄水溶性肽（YPEP）对去卵巢（OVX）大鼠骨代谢的影响，以期替代当前的治疗药物。
在第一步中，研究了 YPEP 对骨丢失的体外作用。测量了前成骨细胞 MC3T3-E1 细胞的增殖、胶原蛋白含量和碱性磷酸酶活性，以及骨髓源性前体细胞的成骨细胞分化。体内实验证实了 YPEP 对骨组织的积极作用。将 3 月龄雌性 Sprague-Dawley 大鼠行假手术或卵巢切除术，并给予商业常规饲料或 0.1%YPEP 补充饲料喂养 3 个月。
YPEP 以剂量依赖性方式增加前成骨细胞 MC3T3-E1 细胞的增殖和碱性磷酸酶活性。胶原蛋白含量也因 YPEP 处理而增加。此外，YPEP 强烈抑制骨髓源性前体细胞的成骨细胞分化。YPEP（100μg/mL）可消除抗酒石酸酸性磷酸酶阳性的破骨细胞的形成。用 YPEP 补充的 OVX 大鼠表现出骨保护作用，因为胫骨的骨矿物质密度和皮质厚度增加，与 OVX 对照组相比。此外，组织学数据表明，YPEP 可防止去卵巢引起的松质骨丢失。在酪蛋白处理的大鼠中未观察到这些保护作用。
本研究表明，YPEP 是治疗骨质疏松症的一种有前途的替代当前治疗药物的方法。
