Molecular Biology and Bioinformatics Laboratory, Department of Genetics and Plant Breeding, Chaudhary Charan Singh University, Meerut, UP, 200054, India.
Biotechnol Lett. 2011 Mar;33(3):629-36. doi: 10.1007/s10529-010-0475-6. Epub 2010 Nov 25.
A draft sequence of the genome of Brachypodium distachyon, the emerging grass model, was recently released. This represents a unique opportunity to determine its functional diversity compared to the genomes of other model species. Using homology mapping of assembled expressed sequence tags with chromosome scale pseudomolecules, we identified 128 alternative splicing events in B. distachyon. Our study identified that retention of introns is the major type of alternative splicing events (53%) in this plant and highlights the prevalence of splicing site recognition for definition of introns in plants. We have analyzed the compositional profiles of exon-intron junctions by base-pairing nucleotides with U1 snRNA which serves as a model for describing the possibility of sequence conservation. The alternative splicing isoforms identified in this study are novel and represent one of the potentially biologically significant means by which B. distachyon controls the function of its genes. Our observations serve as a basis to understand alternative splicing events of cereal crops with more complex genomes, like wheat or barley.
拟南芥的基因组草图最近公布，它是一种新兴的草模型。与其他模式物种的基因组相比，这代表了确定其功能多样性的独特机会。通过组装的表达序列标签与染色体规模的假基因同源映射，我们在拟南芥中鉴定出 128 个可变剪接事件。我们的研究表明，内含子的保留是该植物中主要的可变剪接事件类型（53%），并强调了植物中剪接位点识别定义内含子的普遍性。我们通过与 U1 snRNA 配对的碱基对分析了外显子-内含子接头的组成分布，U1 snRNA 是描述序列保守性可能性的模型。本研究中鉴定的可变剪接异构体是新的，代表了拟南芥控制其基因功能的潜在生物学意义的一种手段。我们的观察结果为理解具有更复杂基因组的谷类作物（如小麦或大麦）的可变剪接事件提供了基础。
