Department of Animal Sciences, Chungbuk National University, Gaesin-dong, Cheongju, Chungbuk, 361-763, South Korea.
J Assist Reprod Genet. 2011 Apr;28(4):325-33. doi: 10.1007/s10815-010-9509-1. Epub 2010 Nov 25.
To evaluate DNA synthesis and epigenetic modification in mouse oocytes during the first cell cycle following the injection of human or hamster sperm.
Mouse oocytes following the injection of human and hamster sperm and cultured in M16 medium.
Male and female pronucleus formation, DNA synthesis, histone protein modification, and heterochromatin formation were similar in mouse oocytes injected with human or hamster sperm. However, DNA methylation patterns were altered in mouse oocytes following human sperm injection. Immunocytochemical staining with a histone H3-MeK9 antibody revealed that human and hamster sperm chromatin associated normally with female mouse chromatin, then entered into the metaphase and formed normal, two-cell stage embryos.
Although differences in epigenetic modification of DNA were observed, fertilization and cleavage occurred in a species non-specific manner in mouse oocytes.
评估在注射人或仓鼠精子后，小鼠卵母细胞在第一个细胞周期中的 DNA 合成和表观遗传修饰。
将人或仓鼠精子注入小鼠卵母细胞后，在 M16 培养基中培养。
注入人或仓鼠精子的小鼠卵母细胞核和原核的形成、DNA 合成、组蛋白修饰和异染色质形成均相似。然而，人精子注射后的小鼠卵母细胞的 DNA 甲基化模式发生改变。用组蛋白 H3-MeK9 抗体进行免疫细胞化学染色显示，人和仓鼠精子染色质与雌性小鼠染色质正常结合，然后进入中期并形成正常的两细胞期胚胎。
尽管在 DNA 的表观遗传修饰上存在差异，但在小鼠卵母细胞中，受精和卵裂以种属非特异性的方式发生。
