Takahashi M, Oshimi K, Mizoguchi H
Department of Medicine, Tokyo Women's Medical College, Japan.
Exp Hematol. 1990 Jun;18(5):395-9.
We previously demonstrated that human granulocyte-macrophage colony (granulocyte-macrophage colony-forming units, CFU-GM) formation was inhibited by interleukin 2 (IL-2)-treated lymphocytes and their conditioned medium (CM). In the present study, the mechanism of this suppression was investigated. When anti-interferon (IFN)-gamma antibody or anti-tumor necrosis factor (TNF)-alpha antibody was added to CFU-GM agar culture with IL-2-treated lymphocytes or their CM, the inhibition of CFU-GM colony formation was partially abrogated, whereas anti-TNF-beta antibody did not abolish the inhibitory effects. When anti-IFN-gamma and anti-TNF-alpha antibodies were added simultaneously, full recovery of colony formation was observed. In the CM of IL-2-treated lymphocytes, detectable levels of IFN-gamma (81 +/- 15 U/ml) and TNF (3.1 +/- 1.1 U/ml) were found. Addition of IFN-gamma and TNF-alpha at these concentrations to the agar culture inhibited CFU-GM colony formation. Taken together, these results indicate that inhibition of human CFU-GM colony formation by IL-2-treated lymphocytes and their CM is mediated by IFN-gamma and TNF-alpha generated from IL-2-treated lymphocytes.
我们之前证明，白细胞介素2（IL-2）处理的淋巴细胞及其条件培养基（CM）可抑制人粒细胞-巨噬细胞集落（粒细胞-巨噬细胞集落形成单位，CFU-GM）的形成。在本研究中，对这种抑制机制进行了研究。当将抗干扰素（IFN）-γ抗体或抗肿瘤坏死因子（TNF）-α抗体添加到含IL-2处理的淋巴细胞或其CM的CFU-GM琼脂培养物中时，CFU-GM集落形成的抑制作用部分被消除，而抗TNF-β抗体并未消除抑制作用。当同时添加抗IFN-γ和抗TNF-α抗体时，观察到集落形成完全恢复。在IL-2处理的淋巴细胞的CM中，检测到可检测水平的IFN-γ（81±15 U/ml）和TNF（3.1±1.1 U/ml）。将这些浓度的IFN-γ和TNF-α添加到琼脂培养物中可抑制CFU-GM集落形成。综上所述，这些结果表明，IL-2处理的淋巴细胞及其CM对人CFU-GM集落形成的抑制作用是由IL-2处理的淋巴细胞产生的IFN-γ和TNF-α介导的。
