Iizuka Y, Endo M, Horikoshi A, Takeuchi J, Ohshima T
First Department of Internal Medicine, Nihon University School of Medicine, Tokyo, Japan.
Exp Hematol. 1990 Feb;18(2):119-23.
A supernatant obtained from the culture of recombinant human interleukin 2-activated lymphocytes was added to granulocyte (G)-, macrophage (M)-, and granulocyte-macrophage (GM)-progenitor assays using a plasma clot culture method. The supernatant was found to exert a suppressive influence on the colony and cluster formations of the G progenitors. This suppressive influence of the supernatant was almost completely neutralized by the addition of a combination of anti-human tumor necrosis factor alpha (HuTNF-alpha) and anti-human interferon gamma (HuIFN-gamma) antibodies, and it has been speculated that this suppressive influence of the supernatant is probably due to TNF-alpha and IFN-gamma. The numbers of M and GM progenitors increased remarkably in the assays in which the supernatant was added when compared to assays without the supernatant; this suggests that these progenitors were resistant to TNF-alpha and IFN-gamma. Among the bone marrow mononuclear cells (BMNC) excluding erythroid-rosette-forming (ERFC) or adherent cells, the influence of the supernatant on these progenitors was not significantly different. The results of our study have shown that ERFC and the adherent cells in BMNC hardly affected the appearance of the suppressive influence.
使用血浆凝块培养法，将重组人白细胞介素2激活的淋巴细胞培养物获得的上清液添加到粒细胞（G）、巨噬细胞（M）和粒细胞-巨噬细胞（GM）祖细胞测定中。发现该上清液对G祖细胞的集落和集簇形成有抑制作用。通过添加抗人肿瘤坏死因子α（HuTNF-α）和抗人干扰素γ（HuIFN-γ）抗体的组合，该上清液的这种抑制作用几乎完全被中和，并且据推测，该上清液的这种抑制作用可能归因于TNF-α和IFN-γ。与未添加上清液的测定相比，在添加上清液的测定中，M和GM祖细胞的数量显著增加；这表明这些祖细胞对TNF-α和IFN-γ具有抗性。在排除红细胞玫瑰花结形成（ERFC）细胞或贴壁细胞的骨髓单个核细胞（BMNC）中，上清液对这些祖细胞的影响没有显著差异。我们的研究结果表明，BMNC中的ERFC细胞和贴壁细胞几乎不影响抑制作用的表现。
