Dermatology Department, Clinic for Small Animal internal Medicine, Vetsuisse Faculty, Winterthurerstrasse 260, CH-8057 Zurich, Switzerland.
Vet Microbiol. 2011 Apr 21;149(1-2):85-90. doi: 10.1016/j.vetmic.2010.10.019. Epub 2010 Nov 4.
The number of recognized papillomavirus (PV) species and potential PV genera has dramatically been increasing throughout the past decade. It seems that every host species might potentially harbour a large set of PVs, while the PVs of each species appear to belong to only a few genera. In horses at least three conditions beside the equine sarcoid have been described, being supposedly PV induced namely classical equine papillomas, genital papillomas and aural plaques. We were able to identify the DNA of novel equine PVs (EcPVs) in the two latter disorders where PV involvement had been predicted. Both PV genomes were entirely cloned and sequenced. Both EcPV genomes, one derived from a penile papilloma, the other derived from an ear papilloma contain the characteristic open reading frames (ORFs) E6, E7, E1, E2, L2 and L1, a large non-coding region between the late and early region as well as a small non-coding region between the early and the late region. The viruses were consequently designated as EcPV2 and EcPV3. The genomes of the three equine PVs were analysed and compared with each other and further PVs. Upon phylogenetic analyses the equine PVs group well together. Pairwise alignment of the L1 nucleotide sequences reveals that EcPV1 shares 54.9% identities with EcPV2 and 53.2% with EcPV3. EcPV2 and EcPV3 share 51.3% identities. As the three EcPVs share less than 60% of nucleotide identities in L1, they may be regarded as belonging to different genera.
在过去的十年中，已被识别的乳头瘤病毒 (PV) 种属和潜在的 PV 属数量显著增加。似乎每一种宿主物种都可能携带大量的 PV，而每种物种的 PV 似乎只属于少数几个属。在马中，除了马肉瘤外，至少还有三种情况被描述为可能由 PV 引起的疾病，即典型的马乳头瘤、生殖器乳头瘤和耳部斑块。我们能够在预测到 PV 参与的后两种疾病中鉴定出新型马乳头瘤病毒 (EcPV) 的 DNA。这两个 PV 基因组都被完全克隆和测序。这两个 EcPV 基因组，一个来自阴茎乳头瘤，另一个来自耳部乳头瘤，都包含特征性的开放阅读框 (ORF) E6、E7、E1、E2、L2 和 L1、晚期和早期区域之间的大非编码区以及早期和晚期区域之间的小非编码区。因此，这两种病毒被命名为 EcPV2 和 EcPV3。对这三种马乳头瘤病毒的基因组进行了分析，并与其他乳头瘤病毒进行了比较。在系统进化分析中，马乳头瘤病毒聚为一组。L1 核苷酸序列的成对比对显示，EcPV1 与 EcPV2 共享 54.9%的同一性，与 EcPV3 共享 53.2%的同一性。EcPV2 和 EcPV3 共享 51.3%的同一性。由于这三种 EcPV 在 L1 中的核苷酸同一性小于 60%，它们可能被视为属于不同的属。
