Department of Veterinary Comparative Anatomy, Pharmacology and Physiology and WWAMI Medical Education Program, Washington State University, Spokane, WA 99210-1945, United States.
Neurosci Lett. 2011 Mar 3;490(3):165-9. doi: 10.1016/j.neulet.2010.11.034. Epub 2010 Nov 24.
Electroencephalographic slow wave activity (SWA) during slow wave sleep (SWS) undergoes dynamic fluctuations in reaction to sleep/wake history. SWA increases as a consequence of prior waking and decreases as consequence of prior SWS. These fluctuations are evidence for a homeostatic regulatory process, the neurobiological underpinnings of which remain to be defined. The anti-neuroinflammatory agent minocycline abolishes the increase in SWA that normally occurs after 1- or 3-h sleep deprivation. We sought to determine whether this effect is also observed during spontaneous sleep. We describe a novel procedure for measuring the predictive relationship between spontaneous changes in sleep/wake states in the short-term (less than 30 min) and subsequent SWA. In saline-treated mice, 16 or more minutes of spontaneous wakefulness during a 20-min interval causes an increase in SWA during subsequent SWS, and 16 or more minutes spent in SWS causes a decrease in SWA during subsequent SWS. Minocycline administration (45 mg/kg) abolishes the increase caused by wakefulness but not the decrease caused by sleep. These data demonstrate that minocycline attenuates SWA dynamics in spontaneous sleep. Inflammatory events in the brain may underlie, in part, wakefulness-induced changes in the sleep electroencephalogram.
脑电图慢波活动（SWA）在慢波睡眠（SWS）期间会对睡眠/觉醒历史做出动态波动。SWA 会随着先前的清醒而增加，随着先前的 SWS 而减少。这些波动证明了一个稳态调节过程，其神经生物学基础仍有待确定。抗炎药物米诺环素消除了 1 或 3 小时剥夺睡眠后 SWA 通常会增加的现象。我们试图确定这种效应是否也会在自发性睡眠期间观察到。我们描述了一种新的方法来测量睡眠/觉醒状态在短时间内（小于 30 分钟）的自发性变化与随后的 SWA 之间的预测关系。在生理盐水处理的小鼠中，在 20 分钟的间隔内自发清醒 16 分钟或更长时间会导致随后的 SWS 期间 SWA 增加，而在 SWS 中度过 16 分钟或更长时间会导致随后的 SWS 中 SWA 减少。米诺环素（45mg/kg）给药消除了清醒引起的增加，但不能消除睡眠引起的减少。这些数据表明，米诺环素减弱了自发性睡眠中的 SWA 动力学。大脑中的炎症事件可能部分导致了睡眠脑电图中清醒诱导的变化。
