CESAM (Centre for Environmental and Marine Studies) & Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
Talanta. 2010 Dec 15;83(2):505-12. doi: 10.1016/j.talanta.2010.09.050. Epub 2010 Oct 27.
Rainwater is a very low concentrated matrix and, for dissolved organic matter (DOM) characterization, an efficient extraction procedure is essential. Isolation procedures based on the adsorption onto XAD-8 and C-18 sorbents have been used in the literature for rainwater DOM isolation, but a comparison between these procedures is lacking. In this work, UV-visible and molecular fluorescence spectroscopies highlighted differences between rainwater DOM isolated by DAX-8 (replacement for XAD-8) and by C-18. It was possible to recover higher rainwater DOM percentage by the C-18 based procedure than by the DAX-8 one. Rainwater protein-like compounds were better concentrated by the C-18 procedure than by the DAX-8 one, while humic-like compounds were similarly concentrated by both procedures. Furthermore, rainwater DOM extracted by the C-18 procedure was more representative of the global matrix, while DAX-8 preferentially extracted humic-like compounds.
雨水是一种浓度非常低的基质，因此对于溶解有机物（DOM）的特征描述，需要一种有效的提取方法。在文献中，基于 XAD-8 和 C-18 吸附剂的吸附的分离方法已经被用于雨水 DOM 的分离，但缺乏对这些方法的比较。在这项工作中，紫外可见光谱和分子荧光光谱强调了 DAX-8（XAD-8 的替代品）和 C-18 分离的雨水 DOM 之间的差异。通过 C-18 方法分离的雨水 DOM 百分比高于 DAX-8 方法。C-18 方法比 DAX-8 方法更能浓缩雨水蛋白类化合物，而腐殖质类化合物则通过两种方法得到了相似的浓缩。此外，用 C-18 方法提取的雨水 DOM 更能代表整个基质，而 DAX-8 则更倾向于提取腐殖质类化合物。
