Peuravuori Juhani, Koivikko Riitta, Pihlaja Kalevi
Department of Chemistry, University of Turku, Finland.
Water Res. 2002 Nov;36(18):4552-62. doi: 10.1016/s0043-1354(02)00172-0.
Aquatic humic solutes were separated by the non-ionic macroporous XAD-8 and DAX-8 resins and a weakly basic DEAE cellulose anion exchanger from seven different fresh water sources. Synchronous fluorescence spectroscopy was applied for characterization, differentiation and classification of the different humic-solute aggregates. Fluorescence properties verified that humic-solute fractions isolated parallelly with the non-ionic XAD-8 and DAX-8 resins resembled very closely each other speaking strongly for their structural similarities. DAX-8 resin separated ca. 19% more aquatic humic matter than did the analogous XAD-8 resin. It was possible to tentatively differentiate the untreated water samples according to their fluorescent materials. Several distinct classes of chromophores were detected in both DOM and isolated humic fractions by the synchronous technique: lambda(ex)/lambda(em) 280/298, 330/348, 355/373, 400/418, 427/445, 460/478, 492/510 and 516/534 nm.
采用非离子型大孔XAD - 8和DAX - 8树脂以及弱碱性DEAE纤维素阴离子交换剂，从七种不同的淡水水源中分离出水相腐殖质溶质。运用同步荧光光谱法对不同腐殖质溶质聚集体进行表征、区分和分类。荧光特性证实，用非离子型XAD - 8和DAX - 8树脂并行分离得到的腐殖质溶质组分彼此非常相似，这有力地表明了它们结构上的相似性。DAX - 8树脂分离出的水生腐殖质比类似的XAD - 8树脂多约19%。根据未经处理水样中的荧光物质，初步区分这些水样是可行的。通过同步技术在溶解性有机质（DOM）和分离出的腐殖质组分中均检测到几类不同的发色团：激发波长/发射波长为280/298、330/348、355/373、400/418、427/445、460/478、492/510和516/534 nm。
