Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
Vet Microbiol. 2011 Apr 21;149(1-2):230-5. doi: 10.1016/j.vetmic.2010.10.023. Epub 2010 Nov 4.
A cross-sectional study was carried out to assess the prevalence and circulation of bluetongue virus (BTV) in Spanish ibexes (Capra pyrenaica hispanica). A total of 770 sera samples, 380 blood samples and 34 spleen samples were collected between 2006 and 2009 in Andalusia (southern Spain), a region and time period with a wide circulation of BTV in livestock. Thirty-one out of 770 (4.0%; CI(95%): 2.6-5.4) sera samples analyzed by ELISA showed antibodies against BTV. Twenty-four out of 31 seropositive samples were tested against BTV serotypes 1, 4 and 8 by serum neutralization test (SNT). Neutralizing antibodies against BTV-1 and BTV-4 were detected in seven and ten animals, respectively, four of them showed neutralizing antibodies to both serotypes. The animals seropositive to BTV-4 were sampled between 2006 and 2008, while BTV-1 circulation was confirmed in ibexes sampled between 2007 and 2009. None of the ibexes presented neutralizing antibodies against BTV-8. Statistically significant differences were found among regions and years, which is in coincidence with what occurred in domestic ruminants. There were no statistically significant differences between sexes, age classes and habitats (captivity vs. free-living). BTV RNA was not found in any of the 380 blood samples analyzed. However, BTV-1 RNA was detected from spleen in one Spanish ibex from Málaga province in August 2008. This finding evidences the presence of BTV-1 in Spanish ibex in a municipality where BT outbreaks were not detected in domestic ruminants during that period. Results of the present study show that Spanish ibexes were exposed and responded serologically to both BTV-1 and BTV-4. The low seroprevalence obtained suggests that Spanish ibex is not a relevant species in the dissemination of BT. However, the detection of BTV-1 RNA and the presence of seropositive ibexes in areas where BT outbreaks were not detected in livestock, could not exclude a significant role in the epidemiology of BTV in certain areas.
本研究旨在评估西班牙野山羊（Capra pyrenaica hispanica）中蓝舌病病毒（BTV）的流行情况和循环情况。2006 年至 2009 年期间，在西班牙南部的安达卢西亚地区（Andalusia）共采集了 770 份血清样本、380 份血液样本和 34 份脾脏样本。通过 ELISA 分析，31 份血清样本（占 770 份血清样本的 4.0%；95%置信区间[CI]：2.6-5.4）显示出针对 BTV 的抗体。在 31 份血清阳性样本中，有 24 份用血清中和试验（SNT）针对 BTV 血清型 1、4 和 8 进行了检测。在 7 只和 10 只动物中分别检测到针对 BTV-1 和 BTV-4 的中和抗体，其中 4 只动物对两种血清型均具有中和抗体。BTV-4 血清阳性动物是在 2006 年至 2008 年期间采样的，而 BTV-1 的流行则在 2007 年至 2009 年期间采样的野山羊中得到证实。没有一只野山羊呈现出针对 BTV-8 的中和抗体。在地区和年份之间发现了统计学上的显著差异，这与家牛群中发生的情况相符。在性别、年龄组和栖息地（圈养与自由放养）之间没有统计学上的显著差异。在分析的 380 份血液样本中均未发现 BTV RNA。然而，在 2008 年 8 月，来自马拉加省（Málaga）的一只西班牙野山羊的脾脏中检测到了 BTV-1 RNA。这一发现表明，在该地区家牛未检出 BT 的情况下，BTV-1 存在于西班牙野山羊中。本研究结果表明，西班牙野山羊曾接触过 BTV-1 和 BTV-4，并对其产生了血清学反应。获得的低血清阳性率表明，西班牙野山羊在 BT 的传播中并不是一个重要的物种。然而，在未在家畜中检出 BT 爆发的地区检测到 BTV-1 RNA 和存在血清阳性的野山羊，不能排除其在某些地区 BTV 流行病学中的重要作用。
