Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
Food Chem Toxicol. 2011 Feb;49(2):477-84. doi: 10.1016/j.fct.2010.11.028. Epub 2010 Nov 26.
Quinocetone (3-methyl-2-quinoxalinbenzenevinylketo-1,4-dioxide, QCT), a new feed antibacterial agent of quinoxaline-1,4-dioxides family, has been used as an animal growth promoter. However, few data about its potential toxicity in vivo were available. In this study, genotoxicity of QCT and the relationship with oxidative stress were investigated. Balb/c mice with both sexes were administrated with QCT (12000, 6000 and 3000 mg/kg/bw, respectively) by gavage acutely. DNA damage, generation of reactive oxygen species (ROS) and activity of antioxidative system (total antioxidative capacity, glutathione, glutathione peroxidase, superoxide dismutase and catalase) in liver and kidney were determined. Moreover, Pu-erh black tea extract (BTE) was co-administrated with QCT to evaluate its protective effect against QCT-induced genotoxicity. The DNA damage was observed in all the groups treated with single QCT except the liver with dose of 3000 mg/kg/bw. ROS was accumulated and antioxidative system was suppressed both in liver and kidney. However, the DNA damage, as well as the ROS, was decreased, while the activity of antioxidative system was increased in mice after co-administration of QCT and BTE. These data demonstrate that oxidative stress mediated the genotoxicity induced by QCT in vivo. Furthermore, this oxidative DNA damage can be attenuated by pre-supplementation of BTE.
喹乙醇（3-甲基-2-喹喔啉苯乙烯基-1,4-二恶烷酮，QCT），一种新型的喹喔啉-1,4-二恶烷类饲料抗菌剂，被用作动物生长促进剂。然而，体内潜在毒性的数据很少。本研究探讨了 QCT 的遗传毒性及其与氧化应激的关系。雌雄Balb/c 小鼠分别经灌胃给予 QCT（12000、6000 和 3000 mg/kg/bw）。检测肝脏和肾脏中 DNA 损伤、活性氧（ROS）生成和抗氧化系统（总抗氧化能力、谷胱甘肽、谷胱甘肽过氧化物酶、超氧化物歧化酶和过氧化氢酶）的活性。此外，还共同给予普洱黑茶提取物（BTE）以评估其对 QCT 诱导遗传毒性的保护作用。除了 3000 mg/kg/bw 剂量的肝脏外，单独给予 QCT 的所有组均观察到 DNA 损伤。ROS 在肝脏和肾脏中均积累，抗氧化系统被抑制。然而，在共同给予 QCT 和 BTE 后，小鼠的 DNA 损伤以及 ROS 减少，而抗氧化系统的活性增加。这些数据表明，氧化应激介导了 QCT 在体内的遗传毒性。此外，BTE 的预先补充可以减轻这种氧化 DNA 损伤。
