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铜/锌超氧化物歧化酶和锰超氧化物歧化酶在鲤鱼中的分子克隆及差异表达模式。

Molecular cloning and differential expression patterns of copper/zinc superoxide dismutase and manganese superoxide dismutase in Hypophthalmichthys molitrix.

机构信息

Yangtze River Fisheries Research Institute, The Chinese Academy of Fisheries Sciences, Jingzhou, Hubei 434000, China.

出版信息

Fish Shellfish Immunol. 2011 Feb;30(2):473-9. doi: 10.1016/j.fsi.2010.11.003. Epub 2010 Nov 26.

DOI:10.1016/j.fsi.2010.11.003
PMID:21112403
Abstract

Copper/zinc superoxide dismutase (Cu,Zn-SOD) cDNA and manganese superoxide dismutase (Mn-SOD) cDNA were first cloned from silver carp Hypophthalmichthys molitrix using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) method. The open reading frame (ORF) of Cu,Zn-SOD is 465 bp and encodes a 154 amino acids (aa) protein, whereas the ORF of Mn-SOD is 675 bp and encodes a 224 aa protein. Multiple polypeptide sequence alignment showed high identity both of Cu,Zn-SOD (70-87%) and Mn-SOD (80-96%) with the species compared. Both Cu,Zn-SOD and Mn-SOD were detected in heart, brain, liver, kidney, spleen, muscle, gill and blood. Cu,Zn-SOD and Mn-SOD were expressed throughout the embryogenesis, indicating their important roles during embryonic development specially at the cleavage stage. Acute hypoxia suppressed expression of Cu,Zn-SOD and Mn-SOD in liver significantly, up-regulated them in gill relatively, indicating that tissue-specific expression of Cu,Zn-SOD and Mn-SOD is an important stress response adapted to hypoxia.

摘要

铜/锌超氧化物歧化酶（Cu，Zn-SOD）cDNA 和锰超氧化物歧化酶（Mn-SOD）cDNA 首先使用反转录-聚合酶链反应（RT-PCR）和快速扩增 cDNA 末端（RACE）方法从银鲫 Hypophthalmichthys molitrix 中克隆。Cu，Zn-SOD 的开放阅读框（ORF）为 465 bp，编码 154 个氨基酸（aa）蛋白，而 Mn-SOD 的 ORF 为 675 bp，编码 224 aa 蛋白。多种多肽序列比对显示，Cu，Zn-SOD（70-87%）和 Mn-SOD（80-96%）与比较物种具有高度同源性。心脏、大脑、肝脏、肾脏、脾脏、肌肉、鳃和血液中均检测到 Cu，Zn-SOD 和 Mn-SOD。Cu，Zn-SOD 和 Mn-SOD 在整个胚胎发生过程中表达，表明它们在胚胎发育过程中特别是在卵裂期发挥着重要作用。急性缺氧显著抑制肝脏中 Cu，Zn-SOD 和 Mn-SOD 的表达，相对上调鳃中的表达，表明 Cu，Zn-SOD 和 Mn-SOD 的组织特异性表达是对缺氧的重要应激反应。

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