Hegde U, Padhye S, Banerjee M, Vidyasagar P B
Department of Chemistry, University of Poona, Pune.
Indian J Biochem Biophys. 1990 Feb;27(1):5-8.
Inhibition of photosystem II (PS II) activity by 8-hydroxyquinoline (8-HQ) has been investigated in case of spinach chloroplasts and isolated photosystem II particles using the thermoluminescence technique. In presence of 8-HQ, water to methylviologen (MV) photoreduction in isolated chloroplasts is inhibited while the reduction of dichlorophenol indophenol is inhibited in both chloroplasts as well as in photosystem II particles. The activity can be restored fully by addition of diphenylcarbazide (DPC), suggesting that the donor side of water oxidation complex is affected. The changes in the thermoluminescence peaks indicate that the charge recombination processes involving S2 or S3 states of the Kok's cycle are probably affected by 8-HQ treatment.
利用热发光技术，对菠菜叶绿体和分离的光系统II颗粒中8 - 羟基喹啉（8 - HQ）对光系统II（PS II）活性的抑制作用进行了研究。在8 - HQ存在的情况下，分离叶绿体中从水到甲基紫精（MV）的光还原受到抑制，而在叶绿体和光系统II颗粒中，二氯酚靛酚的还原均受到抑制。通过添加二苯卡巴肼（DPC），活性可以完全恢复，这表明水氧化复合体的供体侧受到了影响。热发光峰的变化表明，涉及Kok循环的S2或S3状态的电荷复合过程可能受到8 - HQ处理的影响。
