Semin B K, Ivanov I I, Rubin A B
Department of Biophysics, Faculty of Biology, Moscow State University, USSR.
Gen Physiol Biophys. 1990 Feb;9(1):65-70.
Chloroplasts isolated from pea seadlings grown on water containing 45Ca2+ were treated with local anesthetic tetracaine. Addition of tetracaine inactivated the electron transport activity of donor side photosystem II. This inhibition was accompanied by 45Ca2+ release from the chloroplast membranes as the whole and destroyed by osmotic shock. No such effect was observed when Tris or hydroxylamine were used to inhibit the donor side photosystem II. Upon thermal inactivation of chloroplasts 45Ca2+ release occurred but at temperatures above 80 degrees. The functional role of Ca2+ in photosystem II is discussed.
从在含有45Ca2+的水中生长的豌豆幼苗中分离出的叶绿体，用局部麻醉药丁卡因进行处理。添加丁卡因会使供体侧光系统II的电子传递活性失活。这种抑制伴随着45Ca2+从整个叶绿体膜中释放出来，并被渗透休克破坏。当使用Tris或羟胺抑制供体侧光系统II时，未观察到这种效应。叶绿体热失活时会发生45Ca2+释放，但温度需高于80度。本文讨论了Ca2+在光系统II中的功能作用。
